小麦条锈菌3个看家基因SNP引物的首次报道(英文)

陈万权; 段霞瑜; 刘太国; 高利; 《植物病理学报 》 2014 CSCD

摘要：In order to analyze the population structure of Puccinia striiformis f.sp.tritici(Pst),SNPs primers of Pst were developed from DNA sequences of nine house-keeping genes Chs,Act,Mapk1,Tub,Cdc2,Rd,Sm,Ls and Ef-1α obtained from GenBank.Eight of which were from Pst and one from P.graminis f.sp.tritici(Pgt).Thirteen pairs of primers were designed and screened based on at least 30 isolates of Pst obtained from diverse locations.Three of them were polymorphic namely Mapl351S/Mapl683 A,Cd28S/Cd352 A and Efl37S/Ef531 A.Polymorphic loci analysis based on 149 Pst isolates from 5 provinces indicated that the three primers had good polymorphism.Cd28S/Cd352 A had 8 polymorphic loci,three of them were phylogenetically informative.Efl37S/Ef531 A had 6 polymorphic loci and 4 of them were informative.Mapl351S/Mapl683 A had 8 polymorphic loci and 4 were informative.The 3 primers were used for analyzing Pst population and revealed the ancestral origin,phylogeny relation of haplotypes,genetic differentiation of the population,gene flow,and the evolution and migration relation between Pst populations thereby.The findings indicate that the 3genes SNP primers can be used for Pst population genetic structure analysis.

125个云南小麦生产品种成株期抗条锈病及白粉病评价

陈万权; 段霞瑜; 徐世昌; 于亚雄; 毕云青; 孔铃; 《云南农业科技 》 2012

摘要：条锈病抗性评价采用近年来条锈菌优势小种CYR31、CYR32、CYR33及多小种混合菌系,分小种圃进行接种鉴定;白粉病抗性评价采用白粉菌混合菌系进行接种鉴定,对125个云南小麦生产品种进行成株期抗条锈病及白粉病评价。结果表明,有24%的品种兼抗条锈病及白粉病,7.2%的品种抗白粉病感条锈病,42.4%的品种抗条锈病感白粉病,26.4%的品种感条锈病和白粉病。生产上可首要推广兼抗两病的品种,只抗条锈病或白粉病的品种作为应急候补品种酌情种植,兼感两病的品种应尽快压缩播种面积。

关键词： 云南 小麦品种 条锈病 白粉病 抗性评价

小麦条锈菌DNA微量高效提取及AFLP检测

刘太国; 陈万权; 高利; 周益林; 段霞瑜; 《中国植物病理学会2010年学术年会论文集 》 2010 CSCD

摘要：通过对0.5mg、1.0mg、2.5mg、5.0mg、7.5mg、10.0mg6个菌量梯度的条锈菌夏孢子进行DNA微量提取研究,结果表明:DNA浓度与菌量呈正相关,0.5mg的条锈菌夏孢子可提取到69.0ng/μl浓度的DNA,达到AFLP反应需要的50ng/μl的浓度。用该

关键词： 小麦条锈菌 DNA 夏孢子 菌量 引物组合 菌系 玻璃珠 法能 子可

云南省小麦叶锈菌毒性研究

陈万权; 《云南农业大学学报 》 1998 CSCD

摘要：云南省小麦叶锈菌毒性研究ＲｅｓｅａｒｃｈｅｓｏｎｔｈｅＶｉｒｕｌｅｎｃｅｏｆＰｕｃｉｎｉａＲｅｃｏｎｄｉｔａｉｎＹｕｎｎａｎ毕云青１陈万权２（１云南省农业科学院植保所，昆明６５０２０５）（２中国农业科学院植保所，北京１０００９４）小麦锈病是云南省小麦...