小麦条锈菌3个看家基因SNP引物的首次报道(英文)

陈万权; 段霞瑜; 刘太国; 高利; 《植物病理学报 》 2014 CSCD

摘要：In order to analyze the population structure of Puccinia striiformis f.sp.tritici(Pst),SNPs primers of Pst were developed from DNA sequences of nine house-keeping genes Chs,Act,Mapk1,Tub,Cdc2,Rd,Sm,Ls and Ef-1α obtained from GenBank.Eight of which were from Pst and one from P.graminis f.sp.tritici(Pgt).Thirteen pairs of primers were designed and screened based on at least 30 isolates of Pst obtained from diverse locations.Three of them were polymorphic namely Mapl351S/Mapl683 A,Cd28S/Cd352 A and Efl37S/Ef531 A.Polymorphic loci analysis based on 149 Pst isolates from 5 provinces indicated that the three primers had good polymorphism.Cd28S/Cd352 A had 8 polymorphic loci,three of them were phylogenetically informative.Efl37S/Ef531 A had 6 polymorphic loci and 4 of them were informative.Mapl351S/Mapl683 A had 8 polymorphic loci and 4 were informative.The 3 primers were used for analyzing Pst population and revealed the ancestral origin,phylogeny relation of haplotypes,genetic differentiation of the population,gene flow,and the evolution and migration relation between Pst populations thereby.The findings indicate that the 3genes SNP primers can be used for Pst population genetic structure analysis.

小麦条锈菌DNA微量高效提取及AFLP检测

刘太国; 陈万权; 高利; 周益林; 段霞瑜; 《中国植物病理学会2010年学术年会论文集 》 2010 CSCD

摘要：通过对0.5mg、1.0mg、2.5mg、5.0mg、7.5mg、10.0mg6个菌量梯度的条锈菌夏孢子进行DNA微量提取研究,结果表明:DNA浓度与菌量呈正相关,0.5mg的条锈菌夏孢子可提取到69.0ng/μl浓度的DNA,达到AFLP反应需要的50ng/μl的浓度。用该

关键词： 小麦条锈菌 DNA 夏孢子 菌量 引物组合 菌系 玻璃珠 法能 子可