Molecular characterization of wild tomato mosaic virus and chilli veinal mottle virus mix-infecting chilli pepper in China
文献类型： 外文期刊
作者： Hu, Yongliang 1 ; Chen, Yuqin 1 ; Su, Xiaoxia 2 ; Huang, Jiawei 1 ; Yin, Hongxing 1 ; Zhang, Jie 2 ; Zhang, Zhongkai 2 ; Ding, Yong 3 ; Zheng, Kuanyu 2 ;
作者机构： 1.Dehong Trop Agr Res Inst Yannan, Ruili, Peoples R China
2.Biotechnol & Germplasm Resources Inst, Yunnan Acad Agr Sci, Yunnan Prov Key Lab Agr Biotechnol, Kunming, Peoples R China
3.Southwest Forestry Univ, Key Lab Forest Biotechnol Yunnan, Kunming, Peoples R China
4.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Yunnan Prov Key Lab Agr Biotechnol, 2238 Beijing Rd, Kunming 650205, Yunnan, Peoples R China
5.Southwest Forestry Univ, Key Lab Forest Biotechnol Yunnan, 300 Bailongsi Kunming 650224, Yunnan, Peoples R China
关键词： chilli veinal mottle virus; mix-infection; phylogenetic analysis; wild tomato mosaic virus
期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.826； 五年影响因子：1.801 ）
ISSN： 0931-1785
年卷期：
页码：
收录情况： SCI
摘要： The present study described molecular characterization of two potyviruses: wild tomato mosaic virus Dehong isolate (WTMV-Dh) and chilli veinal mottle virus Dehong isolate (ChiVMV-Dh) mix-infection chilli pepper and assessed their mix-infection on chilli pepper. Transcriptome next-generation sequencing technology (NGS) and RT-PCR were used to obtain the complete genome sequences of the abovementioned two viruses. The results showed that the complete sequence of WTMV-Dh is 9675 nucleotides (nt) in length and encodes a complete polyprotein of 3075 amino acids (aa). The WTMV-Dh polyprotein shares 76.1%-82.6% nt and 85.3%-89.5% aa identities with three previously reported WTMV isolates. The complete sequence of ChiVMV-Dh is 9736 nt in length and encodes a complete polyprotein with 3, 089 aa. ChiVMV-Dh polyprotein shares 80.8%-92.2% nt and 85.3%-95.6% aa identities with previously reported ChiVMV isolates. Following phylogenetic analysis based on the polyprotein sequences of potyviruses revealed that WTMV-Dh clustered with the Vietnam strain WTMV-Laichau, while ChiVMV-Dh clustered with several ChiVMV Sichuan isolates. The mix-inoculation experiment showed that the mix-infection of these two viruses has more severe symptoms than single-infection.
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