Molecular characterization of wild tomato mosaic virus and chilli veinal mottle virus mix-infecting chilli pepper in China
文献类型： 外文期刊
作者： Hu, Yongliang 1 ; Chen, Yuqin 1 ; Su, Xiaoxia 2 ; Huang, Jiawei 1 ; Yin, Hongxing 1 ; Zhang, Jie 2 ; Zhang, Zhongkai 2 ; Ding, Yong 3 ; Zheng, Kuanyu 2 ;
作者机构： 1.Dehong Trop Agr Res Inst Yannan, Ruili, Peoples R China
2.Biotechnol & Germplasm Resources Inst, Yunnan Acad Agr Sci, Yunnan Prov Key Lab Agr Biotechnol, Kunming, Peoples R China
3.Southwest Forestry Univ, Key Lab Forest Biotechnol Yunnan, Kunming, Peoples R China
4.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Yunnan Prov Key Lab Agr Biotechnol, 2238 Beijing Rd, Kunming 650205, Yunnan, Peoples R China
5.Southwest Forestry Univ, Key Lab Forest Biotechnol Yunnan, 300 Bailongsi Kunming 650224, Yunnan, Peoples R China
关键词： chilli veinal mottle virus; mix-infection; phylogenetic analysis; wild tomato mosaic virus
期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.826； 五年影响因子：1.801 ）
ISSN： 0931-1785
年卷期：
页码：
收录情况： SCI
摘要： The present study described molecular characterization of two potyviruses: wild tomato mosaic virus Dehong isolate (WTMV-Dh) and chilli veinal mottle virus Dehong isolate (ChiVMV-Dh) mix-infection chilli pepper and assessed their mix-infection on chilli pepper. Transcriptome next-generation sequencing technology (NGS) and RT-PCR were used to obtain the complete genome sequences of the abovementioned two viruses. The results showed that the complete sequence of WTMV-Dh is 9675 nucleotides (nt) in length and encodes a complete polyprotein of 3075 amino acids (aa). The WTMV-Dh polyprotein shares 76.1%-82.6% nt and 85.3%-89.5% aa identities with three previously reported WTMV isolates. The complete sequence of ChiVMV-Dh is 9736 nt in length and encodes a complete polyprotein with 3, 089 aa. ChiVMV-Dh polyprotein shares 80.8%-92.2% nt and 85.3%-95.6% aa identities with previously reported ChiVMV isolates. Following phylogenetic analysis based on the polyprotein sequences of potyviruses revealed that WTMV-Dh clustered with the Vietnam strain WTMV-Laichau, while ChiVMV-Dh clustered with several ChiVMV Sichuan isolates. The mix-inoculation experiment showed that the mix-infection of these two viruses has more severe symptoms than single-infection.
- 相关文献
作者其他论文 更多>>
-
AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato
作者：Zhao, Liling;Chen, Yingfang;Xiao, Xingming;Gao, Haiying;Cao, Jiamin;Guo, Zhongxin;Zhao, Liling;Zhang, Zhongkai
关键词：
-
Interspecies/Intergroup Complementation of Orthotospovirus Replication and Movement through Reverse Genetics Systems
作者：Feng, Mingfeng;Chen, Minglong;Yuan, Yulong;Liu, Qinhai;Cheng, Ruixiang;Yang, Tongqing;Li, Luyao;Guo, Rong;Dong, Yongxin;Chen, Jing;Yang, Yawen;Yan, Yuling;Cui, Hongmin;Jing, Dong;Kang, Jinrui;Chen, Shuxian;Li, Jia;Zhu, Min;Tao, Xiaorong;Huang, Changjun;Zhang, Zhongkai;Kormelink, Richard
关键词：plant negative-stranded RNA viruses; reverse genetics system; orthotospovirus; replication; cell-to-cell movement
-
Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth
作者：Jiang, Lihui;Zhang, Xiaoyan;Wu, Lixia;Fu, Qijing;Zhao, Yiting;Lu, Xinqi;Huang, Wuying;Zhao, Jianping;Li, Kuixiu;Zhao, Shuanglu;Han, Li;Zhou, Xuan;Luo, Chongyu;Zhu, Haiyan;Yang, Jing;Huang, Huichuan;He, Xiahong;Du, Yunlong;Jiang, Lihui;Zhang, Xiaoyan;Wu, Lixia;Fu, Qijing;Zhao, Yiting;Zhao, Jianping;Li, Kuixiu;Zhao, Shuanglu;Han, Li;Zhou, Xuan;Luo, Chongyu;Zhu, Haiyan;Yang, Jing;Huang, Huichuan;He, Xiahong;Du, Yunlong;Jiang, Lihui;Zhang, Xiaoyan;Wu, Lixia;Fu, Qijing;Zhao, Yiting;Zhao, Jianping;Li, Kuixiu;Han, Li;Zhou, Xuan;Luo, Chongyu;Zhu, Haiyan;Yang, Jing;Huang, Huichuan;He, Xiahong;Du, Yunlong;Yao, Baolin;Liu, Changning;Wu, Lixia;Zhao, Yiting;Zhao, Yiting;Cao, Yuxin;Zhu, Ruomeng;Zhang, Zhongkai;Zhu, Zhengge;Friml, Jiri;Liu, Changning;Liu, Changning
关键词：auxin; clathrin; endocytosis; Oryza sativa; OsPIN3t; salicylic acid
-
Quantification of plasmodesmata frequency under three-dimensional view using focused ion beam-scanning electron microscopy and image analysis
作者：Deng, Yinlu;Wang, Yaqin;Hong, Jian;Li, Zhenghe;Xie, Li;Deng, Yinlu;Li, Zhenghe;Shang, Weina;Shang, Weina;Zhang, Xiaomin;Hong, Jian;Xie, Li;Guo, Jiansheng;Guo, Jiansheng;Zhang, Zhongkai
关键词：PD quantification; PD frequency; Focused ion beam-scanning electron micro; scopy; Image analysis
-
Impact of leaf trichomes of tomatoes and weeds on the host selection and developmental bioassays of Bemisia tabaci Q and A cryptic species
作者：Shah, Md Mostafizur Rahman;Zhang, Zhongkai;Hu, Jian;Shah, Md Mostafizur Rahman;Gaber, Ahmed;Hossain, Akbar
关键词：B. tabaci Q; B. tabaci A; Host selection; Tomato; Weed; Leaf trichomes
-
Contributions of ectomycorrhizal fungi in a reclaimed poplar forest (Populus yunnanensis) in an abandoned metal mine tailings pond, southwest China
作者：Xiao, Yinrun;Liu, Conglong;Hu, Na;Wang, Bowen;Zhao, Zhiwei;Li, Tao;Xiao, Yinrun;Liu, Conglong;Hu, Na;Wang, Bowen;Li, Tao;Zheng, Kuanyu
关键词：Bovista limosa; Physiological responses; Heavy metal tolerance; Phytoremediation; Compartmentalization
-
Advances and Prospects of Virus-Resistant Breeding in Tomatoes
作者：Shahriari, Zolfaghar;Su, Xiaoxia;Zheng, Kuanyu;Zhang, Zhongkai;Shahriari, Zolfaghar
关键词：virus resistance gene; RNAi; tomato breeding; CRISPR-Cas; genome editing
