文献类型： 外文期刊

作者： Mi, Chao ¹ ; Zhang, Yusong ³ ; Zhao, Yanning ⁴ ; Lin, Liangbin ² ;

作者机构： 1.Xizang Acad Agr & Anim Husb Sci, Agr Res Inst, Lhasa, Peoples R China

2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Peoples R China

3.Yunnan Acad Agr Sci, Ind Crops Res Inst, Kunming, Peoples R China

4.Xizang Acad Agr & Anim Husb Sci, Vegetable Res Inst, Lhasa, Peoples R China

关键词： BnLPP; BnMYB86; Brassica napus L.; low nighttime temperature; oil accumulation; transcriptome

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：6.4； 五年影响因子：5.9 ）

ISSN： 0031-9317

年卷期： 2024 年 176 卷 3 期

页码：

收录情况： SCI

摘要： Rape (Brassica napus L.; AACC) is an important oil-bearing crop worldwide. Temperature significantly affects the production of oil crops; however, the mechanisms underlying temperature-promoted oil biosynthesis remain largely unknown. In this study, we found that a temperature-sensitive cultivar (O) could accumulate higher seed oil content under low nighttime temperatures (LNT,13 degrees C) compared with a temperature-insensitive cultivar (S). We performed an in-depth transcriptome analysis of seeds from both cultivars grown under different nighttime temperatures. We found that low nighttime temperatures induced significant changes in the transcription patterns in the seeds of both cultivars. In contrast, the expression of genes associated with fatty acid and lipid pathways was higher in the O cultivar than in the S cultivar under low nighttime temperatures. Among these genes, we identified 14 genes associated with oil production, especially BnLPP and ACAA1, which were remarkably upregulated in the O cultivar in response to low nighttime temperatures compared to S. Further, a WGCNA analysis and qRT-PCR verification revealed that these genes were mainly regulated by five transcription factors, WRKY20, MYB86, bHLH144, bHLH95, and NAC12, whose expression was also increased in O compared to S under LNT. These results allowed the elucidation of the probable molecular mechanism of oil accumulation under LNT conditions in the O cultivar. Subsequent biochemical assays verified that BnMYB86 transcriptionally activated BnLPP expression, contributing to oil accumulation. Meanwhile, at LNT, the expression levels of these genes in the O plants were higher than at high nighttime temperatures, DEGs (SUT, PGK, PK, GPDH, ACCase, SAD, KAS II, LACS, FAD2, FAD3, KCS, KAR, ECR, GPAT, LPAAT, PAP, DGAT, STERO) related to lipid biosynthesis were also upregulated, most of which are used in oil accumulation.