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Molecular cloning and characterization of a calreticulin cDNA from the pinewood nematode Bursaphelenchus xylophilus

文献类型： 外文期刊

作者： Li, Xundong 1 ; Zhuo, Kan 1 ; Luo, Mei 1 ; Sun, Longhua 4 ; Liao, Jinling 1 ;

作者机构： 1.S China Agr Univ, Lab Plant Nematol, Guangzhou 510642, Guangdong, Peoples R China

2.Yunnan Acad Agr Sci, Yunnan 650205, Peoples R China

3.Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

4.Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore

关键词： cDNA: complementary DNA;open reading frame;polypeptide

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

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收录情况： SCI

摘要： The cloning and characterization of a cDNA encoding a calreticulin from the pinewood nematode Bursaphelenchus xylophilus is described herein. The full-length cDNA (Bx-crt-1) contained a 1200 bp open reading frame that could be translated to a 399 amino acid polypeptide. The deduced protein contained highly conserved regions of a calreticulin gene and had 66.2-70.1% amino acid sequence identity to other calreticulin sequences from nematodes. RNAi, RT-PCR amplification, and southern blot suggest that Bx-crt-1 may be important for the development of B. xylophilus

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