文献类型： 外文期刊

作者： Huang, Fang ¹ ; Yang, Yan-yan ¹ ; Shi, Min ¹ ; Li, Jun-ying ² ; Chen, Zong-qi ³ ; Chen, Fu-shou ³ ; Chen, Xue-xin ¹ ;

作者机构： 1.Zhejiang Univ, Inst Insect Sci, Key Lab Mol Biol Crop Insects & Pathogens, Minist Agr, Hangzhou 310029, Zhejiang, Peoples R China

2.Zhejiang Univ, Ctr Anal & Measurement, Hangzhou 310029, Zhejiang, Peoples R China

3.Yunnan Acad Agr Sci, Inst Agr Environm & Resource, Kunming, Peoples R China

关键词： Plutella xylostella;Hemocyte;Ultrastructure;Phagocytosis;Cellular immunity;Granulocyte

期刊名称：TISSUE & CELL （ 影响因子：2.466； 五年影响因子：2.235 ）

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收录情况： SCI

摘要： The hemocytes of different types encountered in the diamondback moth Plutella xylostella larvae of each instar and the development of the differential hemocytes counts were herein presented. Hemocytes classes/populations characterized based on their affinity with fluorescent dye (acridine orange) and ultrastructural differences comprised the prohemcoytes (<10-16%), plasmatocytes (22-65%), granulocytes (25-72%), oenocytoids (<1-9%), and spherulocytes (<1%). Prohemcoytes were the smallest cells with a comparatively tremendous nucleus. Plasmatocytes and granulocytes occupied the main proportion of total cell numbers. Oenocytoids were in a most stable presence, i.e. rotund in a diameter of 10 mu m and with a nucleus deviated from the central location; however, sometimes with two nuclei which were adjoining with each other. Spherulocytes were rare and only could be observed occasionally. Ultrastructural investigation revealed that hemocytes in the diamondback moth larvae were of the typical model as in the Lepidoptera insect larvae. It is interesting to find that the cell which could phagocytize bacteria in vitro was granulocyte, not the other types of hemocytes, although plasmatocyte was usually declared to participate in this reaction in various previous studies.