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Metabolomics-based screening and chemically identifying abundant stachydrine as quality characteristic of rare Leucosceptrum canum Smith honey

文献类型： 外文期刊

作者： Yan, Sha 1 ; Wang, Xuan 1 ; Zhao, Hongmu 3 ; Lu, Huanxian 3 ; Tian, Wenli 1 ; Wu, Liming 1 ; Xue, Xiaofeng 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China

2.Shanxi Agr Univ, Coll Food Sci & Engn, Taigu 030801, Peoples R China

3.Yunnan Acad Agr Sci, Sericultural & Apicultural Res Inst, Mengzi 661101, Yunnan, Peoples R China

关键词： Honey; Leucosceptrum canum Smith; Metabolomics strategy; Stachydrine

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.52； 五年影响因子：4.942 ）

ISSN： 0889-1575

年卷期： 2022 年 114 卷

页码：

收录情况： SCI

摘要： Leucosceptrum canum Smith honey (LcSH) is a rare and high-value monofloral honey. However, it is difficult to evaluate the authenticity of LcSH since there is little knowledge of its chemical composition. In the present research, we compared LcSH to other common honeys using a metabolomics strategy and screened a molecular feature with a mass of 143.0948 Da that was unique to LcSH. According to HR-MS and NMR spectroscopy, it was identified as stachydrine, which is well known to have a variety of pharmacological activities. The result was confirmed against a reference standard. A UHPLC-MS/MS method was developed to determine its concentration in honey samples. Stachydrine was abundant in LcSH compared to other honeys, ranging from 0.35 mg/g to 0.68 mg/g. Levels of stachydrine were measured 18 months after the initial sampling and did not show significant statistical differences (p < 0.01), suggesting it is chemically stable in LcSH. This study indicates that stachydrine is a useful characteristic component to evaluate and control LcSH authenticity.

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