Cellulase hyperproduction Trichoderma reesei mutant strain BB8 for the bioextraction and bioconversion of ginsenoside from Panax notoginseng
文献类型： 外文期刊
作者： Li, Chengcheng 1 ; Yu, Lijuan 1 ; Miao, Wanting 2 ; Shi, Jing 2 ; Ma, Xiaoshuang 6 ; Li, Minyi 2 ; Zhong, Ying 2 ; Yang, Zhaoxiang 5 ; Xin, Wenfeng 6 ; Xiao, Huining 7 ;
作者机构： 1.Yunnan Acad Agr Sci, Agroprod Proc Res Inst, Kunming 650000, Peoples R China
2.Nanjing Forestry Univ, Sch Light Ind & Food Sci, Nanjing 210037, Peoples R China
3.Nanjing Forestry Univ, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Nanjing 210037, Peoples R China
4.Nanjing Forestry Univ, Inst Ecol Civilisat Construct & Forestry Dev, Int Innovat Ctr Forest Chem & Mat, Nanjing 210037, Peoples R China
5.Yunnan Characterist Plant Extract Lab Co Ltd, Kunming 650106, Yunnan, Peoples R China
6.Wenshan Univ, Coll Notoginseng Med & Pharm, Wenshan 663000, Peoples R China
7.Univ New Brunswick, Dept Chem Engn, Fredericton, NB E3B 5A3, Canada
关键词： Cellulase; Ginsenoside; Bioconversion
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 318 卷
页码：
收录情况： SCI
摘要： Ginsenoside is an important bioactive substance from Panax notoginseng. However, the extraction efficiency of ginsenoside is very low. Here, we constructed a Trichoderma reesei mutant strain BB8 from T. reesei RUT-C30 by overexpression of bgl1gene to achieve a notable high-level production of cellulosic enzymes. Compared with RUT-C30, BB8 displayed the enhanced beta-glucosidase activity as well as other cellulosic enzymes. After treating the P. notoginseng powder with the crude enzymes from BB8, the cell wall structure of P. notoginseng was significantly destroyed and more inner contents were released from the plant cell. More importantly, the major saponins were converted to the minor ginsenosides, compound K (CK) and the active ginsenoside Rd, increasing the beneficial effect of the saponins. The findings from this work advance strain improvement to produce costeffective cellulosic enzymes and represent a potential alternative for the extraction and conversion of the ginsenoside from P. notoginseng without using harmful reagents.
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