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Jasmonate/ethylene- and NaWRKY6/3-regulated Alternaria resistance depends on ethylene response factor 1B-like in Nicotiana attenuata

文献类型： 外文期刊

作者： Ma, Lan 1 ; Song, Na 2 ; Duan, Qing 1 ; Du, Wenwen 1 ; Li, Xiang 1 ; Jia, Wenjie 1 ; Cui, Guangfen 1 ; Wang, Jihua 1 ; Wu, Jinsong 2 ;

作者机构： 1.Yunnan Acad Agr Sci, Natl Engn Res Ctr Ornamental Hort, Yunnan Prov Key Lab Flower Breeding, Yunnan Seed Lab,Flower Res Inst, Kunming 650205, Peoples R China

2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Peoples R China

关键词： Alternaria; ERF; ethylene; jasmonate; Nicotiana; phytoalexin; scopoletin; solavetivone; WRKY

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 0022-0957

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Biosynthesis of the phytoalexins scopoletin and scopolin in Nicotiana species is regulated by upstream signals including jasmonate (JA), ethylene (ET), and NaWRKY3 in response to the necrotrophic fungus Alternaria alternata, which causes brown spot disease. However, how these signals are coordinated to regulate these phytoalexins remains unknown. By analyzing RNA sequencing data and RNAi, we identified NaERF1B-like (NaERF1B-L) as a key player in Nicotiana attenuata during A. alternata infection by regulating the transcripts of Feruloyl-CoA 6'-hydroxylase 1 (NaF6'H1), encoding a key enzyme for scopoletin biosynthesis, and NaVS1-like (NaVS1-L), a putative biosynthetic gene of the phytoalexin solavetivone. We further demonstrated that the synergistic induction of these two genes by JA and ET signaling is mediated by NaERF1B-L. Additionally, we found that the two closely related proteins, NaWRKY6 and NaWRKY3, physically interact to enhance NaERF1B-L expression by directly binding to and activating the NaERF1B-L promoter. Collectively, our current results demonstrate that NaERF1B-L plays a positive role in resistance to A. alternata by modulating phytoalexin biosynthesis through the integration of JA/ET and NaWRKY6/3 signaling. Our findings reveal a fine-tuned transcriptional regulatory hierarchy mediated by NaERF1B-L for brown spot disease resistance in wild tobacco. Ethylene response factor NaERF1B-like integrates signals from jasmonate/ethylene and NaWRKY6/3 to regulate biosynthesis of the phytoalexin scopoletin for brown spot disease resistance to fungal pathogen Alternaria alternata in wild tobacco Nicotiana attenuata .

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