文献类型： 外文期刊

作者： Zhao, Meiai ¹ ; Liu, Shuangshuang ¹ ; Pei, Yuhe ² ; Jiang, Xuwen ² ; Jaqueth, Jennifer S. ³ ; Li, Bailin ³ ; Han, Jing ⁴ ; Jeffers, Daniel ⁵ ; Wang, Jiabo ⁶ ; Song, Xiyun ² ;

作者机构： 1.Qingdao Agr Univ, Coll Life Sci, Key Lab Plant Biotechnol Univ Shandong Prov, Qingdao 266109, Peoples R China

2.Qingdao Agr Univ, Coll Agron, Key Lab Qingdao Major Crop Germplasm Resource Inn, Qingdao 266109, Peoples R China

3.Corteva Agrisci, 7300 NW 62nd Ave, Johnston, IA 50131 USA

4.Shandong Denghai Pioneer, Jinan 254000, Shandong, Peoples R China

5.Yunnan Acad Agr Sci, Yunnan Off, Kunming 650205, Yunnan, Peoples R China

6.Southwest Minzu Univ, Minist Educ, Key Lab Qinghai Tibetan Plateau Anim Genet Resour, Chengdu 160041, Sichuan, Peoples R China

关键词： Maize; MRDD; GWAS; Linkage mapping; Candidate genes

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 315 卷

页码：

收录情况： SCI

摘要： Maize rough dwarf disease (MRDD) is a viral disease that causes substantial yield loss, especially in China's summer planted maize area. Discovery of resistance genes would help in developing high-yielding resistant maize hybrids. Genome-wide association studies (GWASs) have advanced quickly and are now a powerful tool for dissecting complex genetic architectures. In this study, the disease severity index (DSI) of 292 maize inbred lines and an F6 linkage population were investigated across multiple environments for two years. Using the genotypes obtained from the Maize SNP 50K chip, a GWAS was performed with four analytical models. The results showed that 22 SNPs distributed on chromosomes 1, 3, 4, 6, 7 and 8 were significantly associated with resistance to MRDD (P<0.0001). The SNPs on chromosomes 3, 6 and 8 were consistent with the quantitative trait locus (QTL) regions from linkage mapping in an RIL population. Candidate genes identified by GWAS included an LRR receptor-like serine/threonine-protein kinase (GRMZM2G141288), and a DRE-binding protein (GRMZM2G006745). In addition, we performed an allele variation analysis of the SNP loci selected by GWAS and linkage mapping and found that the main alleles of the two SNP loci PZE_101170408 and PZE_106082685 on chromosome 1 differed in terms of disease-resistant materials and disease-susceptible materials. The identified SNPs and genes provide useful information for MRDD-related gene cloning and insights on the underlying disease resistance mechanisms, and they can be used in marker-assisted breeding to develop MRDD-resistant maize.