Sugarcane Pan-Transcriptome Identifying a Master Gene ScHCT Regulating Lignin and Sugar Traits
文献类型： 外文期刊
作者： Chen, Meiyan 1 ; Liu, Peng 1 ; An, Ruilin 1 ; He, Xinhua 1 ; Zhao, Peifang 2 ; Huang, Dongliang 4 ; Yang, Xiping 1 ;
作者机构： 1.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530005, Peoples R China
2.Natl Key Lab Biol Breeding Trop Crops, Kunming 650221, Peoples R China
3.Yunnan Acad Agr Sci, Sugarcane Res Inst, Yunnan Key Lab Sugarcane Genet Improvement, Kaiyuan 661699, Peoples R China
4.Guangxi Acad Agr Sci, Sugarcane Res Inst, Key Lab Sugarcane Biotechnol & Genet Improvement G, Minist Agr & Rural Affairs,Guangxi Key Lab Sugarca, Nanning 530007, Peoples R China
关键词： pan-transcriptome; ScHCT; sugar; lignin; sugarcane
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 2 期
页码：
收录情况： SCI
摘要： Sugarcane has the most complex polyploid genome in the world, and sugar-related traits are one of the most important aims in sugarcane breeding. It is essential to construct a representative pan-transcriptome that contains all transcripts of a species for studies on genetic diversity, population expression, and omics analyses in sugarcane. In this study, we constructed the first comprehensive pan-transcriptome for sugarcane, and 8434 highly reliable open reading frames were found, which were not aligned with any published sugarcane genome. The core and dispensable gene clusters, as well as high- and low-expression gene clusters of the pan-transcriptome, were identified and analyzed. The integration of two sugar content differential transcriptome data revealed nine key candidate genes, including the ScHCT gene, encoding a crucial enzyme for lignin synthesis. Furthermore, the function of the ScHCT gene was validated inArabidopsis, which was negatively correlated with sugar content and positively correlated with lignin content. The interaction protein of ScHCT, ScABH, was screened via a yeast two-hybrid assay and further validated by point-to-point Y2H and bimolecular fluorescence complementation assays. The phenotype of the Arabidopsis abh mutant line revealed that loss of function of ABH resulted in a decrease of sucrose content in stem tissue. This study provides important reference information and genetic resources for sugarcane research and varietal improvement.
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