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Identification and localization of Nup170 in the microsporidian Nosema bombycis

文献类型： 外文期刊

作者： Shang, Ruisha 1 ; Zhu, Feng 2 ; Li, Yu 1 ; He, Ping 1 ; Qi, Jingru 1 ; Chen, Yong 1 ; Sun, Fuzhen 1 ; Zhang, Yiling 1 ; Wang 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Zhenjiang 212018, Jiangsu, Peoples R China

2.Zaozhuang Univ, Coll Life Sci, Zaozhuang 277160, Shandong, Peoples R China

3.Yunnan Acad Agr Sci, Inst Sericulture & Apiculture, Mengzi 661101, Yunnan, Peoples R China

4.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Jiangsu, Peoples R China

关键词： Nosema bombycis; Nuclear pore complex; Nucleoporins; Immunofluorescence; RNA interference

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：1.641； 五年影响因子：1.843 ）

ISSN： 0932-0113

年卷期：

页码：

收录情况： SCI

摘要： As one of the core framework proteins of nuclear pore complex (NPC), nucleoporin Nupl70 acts as a structural adapter between the nucleolus and nuclear pore membrane and maintains the stability of NPC structure through interaction with other proteins. In this study, we identified a Nup170 protein in the microsporidian Nosema bombycis for the first time and named it as NbNup170. Secondary structure prediction showed that the NbNup170 contains alpha-helices and random coils. The three-dimensional structure of NbNup170 is elliptical in shape. Phylogenetic analysis based on the Nup170 and homologous sequences showed that N. bombycis clustered together with Vairimorpha ceranae and Vairimorpha apis. The immunofluorescence localization results showed that the NbNup170 was located on the plasma membrane of the dormant spore and transferred to the surface of sporoplasm in a punctate pattern when the dormant spore has finished germination, and that NbNup170 was distributed on the nuclear membrane and both sides of the nuclei of early proliferative phase, and only on the nuclear membrane during sporogonic phase in the N. bombycis. qPCR analysis showed that the relative expression level of NbNup170 maintained at a low level from 30 to 78 h post-infection with N. bombycis, then reached the highest at 102 h, while that of NbNup170 was repressed at a very low level throughout its life cycle by RNA interference. These results suggested that NbNup170 protein is involved in the proliferative phase and active during the sporogonic phase of N. bombycis.

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