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QTL mapping of physiological traits at the booting stage in rice under low temperature combined with nitrogen fertilization

文献类型: 外文期刊

作者: Yang, Shu Ming 1 ; Zhang, Fei Fei 1 ; Zhang, Su Hua 1 ; Li, Gui Yong 4 ; Zeng, Li Qiong 1 ; Liu, Guan Suo 1 ; Yu, Xiao 1 ;

作者机构: 1.Yunnan Acad Agr Sci, Biotechnol & Genet Resources Inst, Kunming, Yunnan, Peoples R China

2.Minist Agr, Key Lab Southwestern Crop Gene Resources & Germpl, Kunming, Yunnan, Peoples R China

3.Agr Biotechnol Key Lab Yunnan Prov, Kunming, Yunnan, Peoples R China

4.Yunnan Acad Agr Sci, Food Crops Res Inst, Kunming, Yunnan, Peoples R China

5.Yunnan Acad Agr Sci, Inst Agr Environm & Resources, Kunming, Yunnan, Peoples R China

关键词: antioxidant enzymes; cold stress; japonica rice (Oryza sativa L.); nitrogen; QTLs

期刊名称:CZECH JOURNAL OF GENETICS AND PLANT BREEDING ( 影响因子:0.865; 五年影响因子:1.017 )

ISSN: 1212-1975

年卷期: 2019 年 55 卷 4 期

页码:

收录情况: SCI

摘要: Further dissection of physiological molecular mechanisms is indispensable to alleviate rice yield losses resulting from cold injury. By using 105 near-isogenic lines (NILs) derived from a backcross between cv. Lijiangxintuanheigu (LTH) and cv. Towada, we detected quantitative trait loci (QTLs) for physiological traits of the rice flag leaf, based on polymorphic simple sequence repeat (SSR) markers, inclusive composite interval mapping (ICIM), mixed composite interval mapping (MCIM) approaches and phenotypic value subjected to combine with cold-water stress and three nitrogen application rates. By using ICIM, a total of 34 QTLs with additive effects (A-QTLs) were identified on chromosomes 1, 3, 4, 5, 6, 7 and 10, and the phenotypic variation (R-2) explained by each QTL ranged from 8.46 to 29.14%. By using MCIM, 20 A-QTLs and 14 pairs of QTLs with epistatic x environment interaction effects (Epistatic QTLs) were detected, the contribution of environment interaction (H(2)AE) was 0.87 to 7.36%, while the contribution rates of E-QTL, were from 0.97 to 3.58%. Fourteen A-QTLs were detected by ICIM and MCIM, which may serve as a basis for fine-mapping and candidate gene studies, and providing strategies for the development of cold-tolerant rice cultivars and nitrogen application to alleviate chilling stress.

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