Liquid-Liquid Microextraction Based on Acid-Base-Induced Deep Eutectic Solvents for Determination of beta-Carotene and Lycopene in Fruit Juices
文献类型: 外文期刊
作者: Li, Hong 1 ; Zhao, Chunxi 2 ; Tian, Hao 1 ; Yang, Yaling 2 ; Li, Wanyi 3 ;
作者机构: 1.Yunnan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Kunming 650200, Yunnan, Peoples R China
2.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China
3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Yunnan, Peoples R China
关键词: Liquid-liquid microextraction; Acid-base induction; Deep eutectic solvents; Fatty acids; Carotenoids
期刊名称:FOOD ANALYTICAL METHODS ( 影响因子:3.366; 五年影响因子:3.07 )
ISSN: 1936-9751
年卷期: 2019 年 12 卷 12 期
页码:
收录情况: SCI
摘要: An uncomplicated, valid, and convenient way for detection of lycopene and beta-carotene in fruit juice samples using acid-base-induced deep eutectic solvent (DES) liquid-liquid microextraction (DES-ABLLME) prior to HPLC was developed. The ternary fatty acid DESs were prepared by C-9:C-10:C-11 (2:1:1), which serve as hydrogen bond donors and hydrogen bond acceptors synchronously. In extraction procedure, NH3.H2O was used as the emulsifier and reacted with DESs with a milk solution, which can significantly increase the extraction efficiency. HCl was used as the de-emulsifier, which could break the state of emulsification. At optimum conditions, the calibration graphs were linear in the concentration range from 0.1 to 100 mu g mL(-1) for lycopene and from 0.025 to 5.00 mu g mL(-1) for beta-carotene. The limits of detection (LODs) were 0.05 and 0.002 mu g mL(-1) for lycopene and beta-carotene, respectively. The RSD values were varied from 1.3 to 4.1%. Finally, the proposed method was applied to analyze beta-carotene and lycopene in fruit juices.
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