Comparison and quantitative analysis of wild and cultivated Macrohyporia cocos using attenuated total refection-Fourier transform infrared spectroscopy combined with ultra-fast liquid chromatography
文献类型： 外文期刊
作者： Wang, Qinqin 1 ; Zuo, Zhitian 1 ; Huang, Hengyu 2 ; Wang, Yuanzhong 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Yunnan, Peoples R China
2.Yunnan Univ Chinese Med, Coll Tradit Chinese Med, Kunming 650500, Yunnan, Peoples R China
关键词： Macrohyporia cocos; Wild and cultivated species; Attenuated total reflectance-Fourier transform infrared spectroscopy; Triterpene; Partial least squares regression
期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）
ISSN： 1386-1425
年卷期： 2020 年 226 卷
页码：
收录情况： SCI
摘要： Dried sclerotium of Macrohyporia cocos is a well-known and widely-consumed traditional Chinese medicine and is also used as dietary supplement. According to the differential treatment between cultivation and wild habitats in the market, the comparison and quantitative analysis of wild and cultivated M. cocos were performed using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy and ultra-fast liquid chromatography combined with partial least squares discriminant analysis and partial least squares regression (PLSR). 636 samples were used for the spectral scan and chromatographic analysis. Results indicated that contents of dehydrotumulosic acid, poricoic add A and dehydrotrametenolic acid in cultivated samples were significantly different from wild samples in two medicinal parts. Differences of dehydropachymic acid and pachymic acid just existed in inner part samples (P < 0.05). Wild M. cocos samples could be discriminated with cultivated samples with >95.14% efficiency using spectral data. ATR-FTIR combined with PLSR provided satisfactory performance for content predictions of poricoic acid A and dehydrotrametenolic acid. This study demonstrated that growth patterns could affect the quality of inner part and epidermis of M. cocos, and ATR-FTIR was a promising technique for the identification of wild and cultivated M. cocos and the rapid determination of triterpene acids contents. (C) 2019 Elsevier B.V. All rights reserved.
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