您好，欢迎访问云南省农业科学院 机构知识库！

Transcriptome analysis of axillary bud differentiation in a new dual-axillary bud genotype of sugarcane

文献类型： 外文期刊

作者： Chen, Dan 1 ; Lu, Xin 2 ; Wu, Xiaoyang 1 ; Ying, Xiongmei 2 ; Long, Wenjie 1 ; Su, Huosheng 2 ; Liu, Hongbo 2 ; Lin, Xiuq 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650205, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Sugarcane Res Inst, Kaiyuan 661699, Peoples R China

关键词： Sugarcane; Dual-axillary buds; RNA-seq; Transcriptome; Differentially expressed genes (DEGs)

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）

ISSN： 0925-9864

年卷期： 2020 年 67 卷 3 期

页码：

收录情况： SCI

摘要： Each node of the sugarcane stem has a single axillary bud. Therefore, to ensure yield, a large number of cane setts are required for planting, which increases production costs. The dual-axillary bud genotype could provide two buds in a same node so as to reduce the amount of cane setts and ensure enough buds when same sowing planted. To studying the molecular characteristics of this genotype, we performed transcriptome sequencing (RNA-seq) analysis with between the dual-axillary bud genotype and its sister line, the single-axillary bud genotype at the axillary bud differentiation stage. A total of 84.78 Gb clean data were obtained, with an average of 10.90 Gb clean data from each genotype. By aligning to the databases, a total of 50,248 unigenes were functionally annotated. 1646 differentially expressed genes (543 up-regulated and 1103 down-regulated genes) were identified between the two genotypes. The expression of genes involved in the carotenoid pathway including F-box proteins, SCF protein complex, and alpha/beta-hydrolase fold enzymes were down-regulated, 9-cis-epoxycarotenoid dioxygenase was up-regulated, the P450 family exhibited both up- and down- regulated. And genes related to shoot branching, including WRKY transcription factors, the Myb-like DNA-binding domain gene family, and bHLH transcription factors showed both up- and down- regulated, the NAC domain gene family, GRAS gene family, bZIP transcription factors, and R2R3-MYB transcription factors all exhibited down-regulated. Overall, we predict that the dual-axillary bud trait in sugarcane may be related to the significant expression of genes involved in the strigolactone pathway, as well as in genes or transcription factors related to shoot branching.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台