文献类型： 外文期刊

作者： Peng, Xiaowei ¹ ; Hu, Xiang ¹ ; Zhang, Yingjun ³ ; Xu, Han ¹ ; Tang, Junrong ¹ ; Zhang, Guiliang ⁴ ; Deng, Jia ¹ ; Kan, Huan ¹ ; Zhao, Ping ¹ ; Liu, Yun ¹ ;

作者机构： 1.Southwest Forestry Univ, Key Lab Forest Resources Conservat & Utilizat Sou, China Minist Educ, Kunming 650224, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Inst Trop Ecoagr, Yuanmou 651300, Peoples R China

3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China

4.Daweishan Natl Nat Reserve Management Bur, Hekou Management Sub Bur, Hekou 661399, Peoples R China

关键词： Camellia fascicularis; Polysaccharides; Extraction optimization; Structural characterization; Antioxidant; Anti-tumor

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 222 卷

页码：

收录情况： SCI

摘要： The ultrasonic-assisted extraction of polysaccharides from Camellia fascicularis (PCF) was optimized using response surface methodology. After separation and purification with DEAE-52 cellulose and Sephadex G-200 glucan gel columns, the purified polysaccharide components of PCFa-1 and PCFc-1 were analyzed for their structural characterization, antioxidant and anti-tumor activities in vitro. The results indicated that liquid to material ratio of 42 mL/g, ultrasonic time of 53 min, ultrasonic temperature of 73.C, and ultrasonic power of 215 W were the optimum extraction conditions for PCF with maximum yields (4.05 %). PCFa-1 and PCFc-1 contained 5.88 % and 9.58 % uronic acid content, with 7.53 and 108.91 kDa of average molecular weights, respectively. The PCFa-1 was mainly constituted of galactose, arabinose, and glucose, while PCFc-1 was primarily composed of arabinose, glucose, galacturonic acid, and rhamnose. Fourier transform infrared spectra revealed that PCFa-1 and PCFc-1 contained typical polysaccharide bands. Scanning electron microscopy showed that the surface of PCFa-1 and PCFc-1 were irregular and clumpy structures. Nuclear magnetic resonance showed that PCFa-1 and PCFc-1 were mainly alpha-glycosidic bond conformation. Furthermore, the PCFc-1 showed better antioxidant capacities than PCFa-1 against hydroxyl, DPPH, and ABTS radicals and exhibited more potent toxicity on A549 and HepG2 cells. These research results suggested that PCF, especially PCFc-1, possesses great potential as natural antioxidants and anti-tumor drugs.