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Identification of BmELP as an interaction partner of Bmtutl-519 in the silkworm, Bombyx mori

文献类型： 外文期刊

作者： Zhu, Feng 1 ; Gao, Jianhua 2 ; Qiao, Keyuan 1 ; Xiao, Shengyan 2 ; Bai, Xingrong 2 ; Kang, Meiling 1 ; Ming, Dongfeng 1 ;

作者机构： 1.Zaozhuang Univ, Coll Life Sci, Zaozhuang 277160, Shandong, Peoples R China

2.Yunnan Acad Agr Sci, Inst Sericulture & Apiculture, Mengzi 661101, Yunnan, Peoples R China

3.Zaozhuang Univ, Coll Food Sci & Pharmaceut Engn, Zaozhuang 277160, Shandong, Peoples R China

关键词： Bombyx mori; Nosema bombycis; Bmtutl-519; BmELP

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2021 年 24 卷 3 期

页码：

收录情况： SCI

摘要： The Turtle protein could act as a signaling receptor or function as a membrane-bound ligand for an unknown receptor. Alternatively, the Turtle protein could function as a co-receptor in a multi-protein receptor complex. Earlier work on the function of Bmtutl-519 suggested that it may act as a cell surface receptor or as a regulatory factor in the infection process of Nosema bombycis. To further investigate the function of Bmtutl-519 in the process of microsporidian infection and the possible regulatory pathways involved, we performed yeast two-hybrid screening on a silkworm midgut cDNA library and identified a Bombyx mori extensin-like protein/BmELP as a binding partner of Bmtutl-519. The interaction between Bmtutl-519 and BmELP was verified by GST pull-down and co-immunoprecipitation assays. Quantitative RT-PCR analysis showed that BmELP was commonly expressed in all of the examined tissues of the silkworm, and the expression was highest in the head. Moreover, BmELP was expressed throughout the entire development period, and the lowest levels of expression were observed at the egg stage. The results of infection experiments showed that N. bombycis infection caused the up-regulation of BmELP expression, especially in vitro. Taken together, our findings may provide new insights into the roles of BmELP and Bmtutl-519 in microsporidian infection.

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