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Structure of the Mating-Type Genes and Mating Systems of Verpa bohemica and Verpa conica (Ascomycota, Pezizomycotina)

文献类型： 外文期刊

作者： Sun, Wenhua 1 ; Liu, Wei 2 ; Cai, Yingli 3 ; Shi, Xiaofei 2 ; Wu, Liyuan 2 ; Zhang, Jin 2 ; Er, Lingfang 2 ; Huang, Qiuchen 2 ; Yin, Qi 1 ; Zhao, Zhiqiang 6 ; He, Peixin 1 ; Yu, Fuqiang 2 ;

作者机构： 1.Zhengzhou Univ Light Ind, Sch Food & Biol Engn, Zhengzhou 450002, Peoples R China

2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Germplasm Bank Wild Species, Kunming 650201, Peoples R China

3.Yunnan Acad Agr Sci, Inst Agroprod Proc, Kunming 650221, Peoples R China

4.Yunnan Agr Univ, Coll Resource & Environm, Kunming 650100, Peoples R China

5.Southwest Forestry Univ, Sch Biodivers Conservat, Kunming 650224, Peoples R China

6.Agr Technol Promot Stn Zhuoni Cty, Gannan 747600, Peoples R China

关键词： fungi; life cycle; homothallism; heterothallism; MAT1-1-1; MAT1-2-1; MAT1-1-10; MAT1-1-11

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 9 卷 12 期

页码：

收录情况： SCI

摘要： Verpa spp. are potentially important economic fungi within Morchellaceae. However, fundamental research on their mating systems, the key aspects of their life cycle, remains scarce. Fungal sexual reproduction is chiefly governed by mating-type genes, where the configuration of these genes plays a pivotal role in facilitating the reproductive process. For this study, de novo assembly methodologies based on genomic data from Verpa spp. were employed to extract precise information on the mating-type genes, which were then precisely identified in silico and by amplifying their single-ascospore populations using MAT-specific primers. The results suggest that the MAT loci of the three tested strains of V. bohemica encompassed both the MAT1-1-1 and MAT1-2-1 genes, implying homothallism. On the other hand, amongst the three V. conica isolates, only the MAT1-1-1 or MAT1-2-1 genes were present in their MAT loci, suggesting that V. conica is heterothallic. Moreover, bioinformatic analysis reveals that the three tested V. bohemica strains and one V. conica No. 21110 strain include a MAT1-1-10 gene in their MAT loci, while the other two V. conica strains contained MAT1-1-11, exhibiting high amino acid identities with those from corresponding Morchella species. In addition, MEME analysis shows that a total of 17 conserved protein motifs are present among the MAT1-1-10 encoded protein, while the MAT1-1-11 protein contained 10. Finally, the mating type genes were successfully amplified in corresponding single-ascospore populations of V. bohemica and V. conica, further confirming their life-cycle type. This is the first report on the mating-type genes and mating systems of Verpa spp., and the presented results are expected to benefit further exploitation of these potentially important economic fungi.

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