文献类型： 外文期刊

作者： Gao, Fuhong ¹ ; Anane, Rex Frimpong ² ; Liu, Zhengling ¹ ; Zi, Shaomei ³ ; Li, Shangyun ³ ; Yang, Zefen ³ ; Chu, Bifan ³ ; Chen, Xingquan ³ ; Chen, Zeli ⁴ ; Zhao, Mingfu ³ ;

作者机构： 1.Yunnan Tobacco Co, Kunming Branch, 523 Beijing Rd, Kunming 650051, Yunnan, Peoples R China

2.Monash Univ, Monash Biomed Discovery Inst, Dept Biochem & Mol Biol, 23 Innovat Walk, Clayton, Vic 3800, Australia

3.Yunnan Agr Univ, Key Lab Agr Biodivers Pest Management China, Minist Educ, 95 Jinhei Rd, Kunming 650201, Yunnan, Peoples R China

4.Yunnan Agr Univ, Coll Agron & Biotechnol, 95 Jinhei Rd, Kunming 650201, Yunnan, Peoples R China

5.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Beijing Rd, Kunming 2238650205, Yunnan, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.7； 五年影响因子：2.4 ）

ISSN： 0304-8608

年卷期： 2023 年 168 卷 10 期

页码：

收录情况： SCI

摘要： Fusarium diseases include wilts, blights, rots, and cankers of many horticultural, field, ornamental, and forest crops in both agricultural and natural ecosystems, and they significantly hinder food plant production. Here, we describe a novel mycovirus, tentatively designated as "Fusarium fusarivirus 1" (FuFV1), which was discovered in an isolate of the phytopathogenic fungus Fusarium sp. FuFV1 has a positive-sense single-stranded RNA (+ssRNA) genome of 6,391 nucleotides (nt) containing three open reading frames (ORFs). ORF1 encodes a large polypeptide of 1,501 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and helicase (Hel) domains. ORF2, overlapping ORF1 by 122 nucleotides, encodes a polypeptide with a conserved Smc domain. The third and smaller ORF (ORF3) encodes a polypeptide with an unknown function. BLASTp analysis of the ORF1-encoded polypeptide revealed that FuFV1 shares the highest aa sequence similarity (68.5% identity, E-value 0.0) with Fusarium poae fusarivirus 1 (FpFV1, genus Alphafusarivirus). Phylogenetic analysis of the RdRp and helicase (Hel) sequences indicated that FuFV1 clustered closely with FpFV1 in a separate branch within the clade containing members of the genus Alphafusarivirus. Based on these results, we propose that FuFV1 should be considered a novel mycovirus belonging to the genus Alphafusarivirus of the family Fusariviridae.