Triplex Immunostrip Assay for Rapid Diagnosis of Tobacco Mosaic Virus, Tobacco Vein Banding Mosaic Virus, and Potato Virus Y
文献类型： 外文期刊
作者： Guo, Yushuang 1 ; Wu, Wei 2 ; Zhang, Xiaolian 1 ; Ding, Ming 4 ; Yu, Jing 1 ; Zhang, Jie 1 ; Jia, Meng-ao 1 ; Tian, Yanping 5 ;
作者机构： 1.Guizhou Acad Tobacco Sci, Key Lab Mol Genet, Guiyang 550081, Peoples R China
2.Fujian Agr & Forestry Univ, Dept Plant Protect, Fuzhou 350002, Peoples R China
3.Guizhou Univ, Coll Life Sci, Key Lab Plant Resources Conservat & Germplasm Inno, Minist Educ, Guiyang 550081, Peoples R China
4.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resource Inst, Kunming, Peoples R China
5.Shandong Agr Univ, Dept Plant Protect, Tai An 271018, Peoples R China
关键词： immunostrip; mixed infection; multiplex assay; rapid diagnosis; virus
期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）
ISSN： 0191-2917
年卷期： 2022 年 106 卷 12 期
页码：
收录情况： SCI
摘要： Mixed virus infection has increasingly become a problem in the production of Solanaceae crops in recent years; therefore, a fast and accurate detection method is needed. In this study, a novel triplex immunostrip assay was developed for the simultaneous detection of tobacco mosaic virus (TMV), tobacco vein banding mosaic virus (TVBMV), and potato virus Y (PVY). The limits of detection of this novel immunostrip reached 200 ppb (ng/ml), 1 ppm (mu g/ml), and 2 ppm for TMV, PVY, and TVBMV particles, respectively. Importantly, no cross-reactivity was observed among TMV, TVBMV, and PVY or to a nontarget virus. When the assay was applied to suspected virus-infected tobacco, tomato, and potato samples collected from fields in Southwest China, samples of single or mixed virus infection were successfully identified. In conclusion, the triplex immunostrip assay provides a fast and easy to use on-site detection method for field epidemiological studies of TMV, TVBMV, and PVY, and for managing diseases that are caused by them.
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