Genotypic variation in the response to embryogenic callus induction and regeneration in Saccharum spontaneum
文献类型: 外文期刊
作者: Li, Chunjia 1 ; Li, Xujuan 1 ; Lin, Xiuqin 1 ; Qin, Wei 1 ; Lu, Xin 1 ; Mao, Jun 1 ; Liu, Xinlong 1 ;
作者机构: 1.Yunnan Acad Agr Sci, Sugarcane Res Inst, Yunnan Key Lab Sugarcane Genet Improvement, Kaiyuan 661699, Peoples R China
关键词: crop wild relative; embryogenic callus; genotypic variation; Saccharum spontaneum; sugarcane; tissue culture
期刊名称:PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION ( 影响因子:1.08; 五年影响因子:1.243 )
ISSN: 1479-2621
年卷期: 2021 年 19 卷 2 期
页码:
收录情况: SCI
摘要: Embryogenic callus induction and regeneration are useful in many aspects of plant biotechnology, especially in the functional characterization of economically important genes. However, in sugarcane, callus induction and regeneration vary across genotypes. Saccharum spontaneum is an important wild germplasm that confers disease resistance and stress tolerance to modern sugarcane cultivars, and its genome has been completely sequenced. The aim of this study was to investigate the effect of genetic variations on embryogenic callus induction and regeneration in S. spontaneum and to screen genotypes having high tissue culture susceptibility. The study was performed using nine genotypes of S. spontaneum and the following five parameters were assessed to determine the response of genotypes to embryogenic callus induction and regeneration: callus induction, embryogenic callus ratio, embryogenic callus induction, embryonic callus regeneration and regeneration capacity. All the genotypes varied significantly (P < 0.01) in all the parameters, except for embryonic callus regeneration, which was high (>80%) for all the genotypes. High broad-sense heritability (86.1-96.8%) indicated that genetic differences are the major source of genotypic variations. Callus induction was found to be strongly positively correlated with embryogenic callus induction (r = 0.890, P < 0.01) and regeneration capacity (r = 0.881, P < 0.01). Among the nine tested genotypes, VN2 was found to be the most responsive to tissue culture and could therefore be used to characterize functional genes in S. spontaneum. We also suggested an approach with potential applications in facilitating the rapid identification of sugarcane genotypes susceptible to tissue culture.
- 相关文献
作者其他论文 更多>>
-
Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses
作者:Kumar, Tanweer;Xu, Chao-Hua;Lu, Xin;Mao, Jun;Lin, Xiu-Qin;Kong, Chun-Yan;Li, Chun-Jia;Li, Xu-Juan;Tian, Chun-Yan;Ebid, Mahmoud H. M.;Liu, Xin-Long;Liu, Hong-Bo;Kumar, Tanweer;Wang, Jun-Gang;Ebid, Mahmoud H. M.
关键词:sugarcane; biotic and abiotic stress; genetic engineering; transgenic sugarcane; genome editing
-
Comparative Analysis of Seven Endogenous Hormones in a Novel Dual-axillary Bud Mutant Genotype of Sugarcane
作者:Wickramasinghe, Kamal Priyananda;Mao, Jun;Xu, Chao-hua;Lin, Xiu-qin;Li, Xu-juan;Qin, Wei;Liu, Hong-bo;Liu, Xin-long;Mehdi, Faisal;Zhao, Pei-fang;Lu, Xin;Wickramasinghe, Kamal Priyananda;Mao, Jun;Xu, Chao-hua;Lin, Xiu-qin;Li, Xu-juan;Qin, Wei;Liu, Hong-bo;Liu, Xin-long;Mehdi, Faisal;Zhao, Pei-fang;Lu, Xin;Wickramasinghe, Kamal Priyananda;Mehdi, Faisal
关键词:Dual-axillary bud; ELISA technique; Endogenous hormones; Sugarcane; YZ 07-86; Zeatin riboside
-
Evaluation of drought tolerance in sugarcane genotypes using the membership function value of drought tolerance (MFVD)
作者:Xu, Chaohua;Mao, Jun;Li, Xujuan;Burner, David M.;Li, Chunjia;Hussin, Shareif H. Hussin H.;Lin, Xiuqin;Liu, Hongbo;Zhao, Peifang;Lu, Xin;Liu, Xinlong
关键词:Saccharum; Yield-related traits; Drought tolerance; MFVD
-
Strigolactones in Sugarcane Growth and Development
作者:Zan, Fenggang;Wu, Zhuandi;Hu, Xin;Feng, Lu;Liu, Xinlong;Liu, Jiayong;Zhao, Liping;Wu, Caiwen;Guo, Jiawen;Wang, Wenzhi;Zhang, Shuzhen
关键词:DWARF27; tilling; sugarcane; strigolactones biosynthesis; ScD27; 2
-
Haplotype variations of sucrose phosphate synthase B gene among sugarcane accessions with different sucrose content
作者:Liu, Hongbo;Lin, Xiuqin;Li, Xujuan;Lu, Xin;Xu, Chaohua;Liu, Xinlong;Liu, Jiayong;Wu, Caiwen;Liu, Hongbo;Luo, Ziliang;You, Qian;Yang, Xiping;Wang, Jianping;Liu, Hongbo;Luo, Ziliang;Wang, Jianping;Liu, Hongbo;Yang, Xiping
关键词:Sugarcane; Sucrose phosphate synthase B (SPSB) gene; Haplotype; Sucrose
-
Genome-wide DNase I-hypersensitive site assay reveals distinct genomic distributions and functional features of open chromatin in autopolyploid sugarcane
作者:Yu, Guangrun;Sun, Bo;Zhu, Zhiying;Teng, Ailing;Han, Jinlei;Zhang, Hui;Wang, Kai;Sun, Bo;Zhu, Zhiying;Mehareb, Eid M.;Liu, Jiayong;Liu, Xinlong;Zhang, Yuebin;Raza, Ghulam;Zhang, Baohong
关键词:sugarcane; open chromatin; DNase I-hypersensitive site; cis-regulatory DNA element; tissue-specific promoter
-
Expression of sucrose metabolizing enzymes in different sugarcane varieties under progressive heat stress
作者:Mehdi, Faisal;Liu, Xinlong;Mehdi, Faisal;Riaz, Zunaira;Mehdi, Faisal;Aman, Afsheen;Galani, Saddia;Liu, Xinlong;Javed, Urooj
关键词:heat stress; sugarcane; sucrose content; thermotolerance potential; sucrose metabolizing enzymes; stress damage indicators