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Genome-wide analysis of the CDPK gene family and their important roles response to cold stress in white clover

文献类型： 外文期刊

作者： Li, Manman 1 ; Chen, Xiuhua 2 ; Huang, Wangqi 3 ; Li, Yanan 4 ; Liu, Qian 4 ; Yan, Wei 4 ; Guo, Changhong 1 ; Shu, Yongjun 1 ;

作者机构： 1.Harbin Normal Univ, Coll Life Sci & Technol, Key Lab Mol Cytogenet & Genet Breeding Heilongjian, Harbin, Heilongjiang, Peoples R China

2.Yunnan Acad Agr Sci, Int Agr Res Inst, Kunming, Yunnan, Peoples R China

3.Yunnan Acad Agr Sci, Flower Res Inst, Kunming, Yunnan, Peoples R China

4.Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Baoshan, Yunnan, Peoples R China

5.Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, 518 Lancheng Rd, Baoshan 678000, Yunnan, Peoples R China

关键词： White clover; CDPK; Genetic regulation network; Cold stress; RNA-seq

期刊名称：PLANT SIGNALING & BEHAVIOR （ 影响因子：2.9； 五年影响因子：2.9 ）

ISSN： 1559-2316

年卷期： 2023 年 18 卷 1 期

页码：

收录情况： SCI

摘要： Calcium-dependent protein kinases (CDPKs) are an important class of calcium-sensitive response proteins that play an important regulatory role in response to abiotic stresses. To date, little is known about the CDPK genes in white clover. White clover is a high-quality forage grass with high protein content, but it is susceptible to cold stress. Therefore, we performed a genome-wide analysis of the CDPK gene family in white clover and identified 50 members of the CDPK genes. Phylogenetic analysis using CDPKs from the model plant Arabidopsis divided the TrCDPK genes into four groups based on their sequence similarities. Motif analysis showed that TrCDPKs within the same group had similar motif compositions. Gene duplication analysis revealed the evolution and expansion of TrCDPK genes in white clover. Meanwhile, a genetic regulatory network (GRN) containing TrCDPK genes was reconstructed, and gene ontology (GO) annotation analysis of these functional genes showed that they contribute to signal transduction, cellular response to stimuli, and biological regulation, all of which are important processes in response to abiotic stresses. To determine the function of TrCDPK genes, we analyzed the RNA-seq dataset and found that most TrCDPK genes were highly up-regulated under cold stress, particularly in the early stages of cold stress. These results were validated by qRT-PCR experiments, implying that TrCDPK genes are involved in various gene regulatory pathways in response to cold stress. Our study may help to further investigate the function of TrCDPK genes and their role in response to cold stress, which is important for understanding the molecular mechanisms of cold tolerance in white clover and improving its cold tolerance.

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