Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21
文献类型： 外文期刊
作者： Munir, Shahzad 1 ; Li, Yongmei 1 ; He, Pengbo 1 ; He, Pengfei 1 ; He, Pengjie 1 ; Cui, Wenyan 1 ; Wu, Yixin 1 ; Li, Xingyu 1 ; Li, Qi 1 ; Zhang, Sixiang 2 ; Xiong, Yangsu 2 ; Lu, Zhanjun 3 ; Wang, Wenbiao 2 ; Zong, Kexian 2 ; Yang, Yongchao 4 ; Yang, Shaocong 5 ; Mu, Chan 5 ; Wen, Heming 4 ; Wang, Yuehu 6 ; Guo, Jun 7 ; Karunarathna, Samantha C. 8 ; He, Yueqiu 1 ;
作者机构： 1.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming, Peoples R China
2.Binchuan Inst Food & Med Inspect & Testing, Binchuan, Peoples R China
3.Gannan Normal Univ, Coll Life Sci, Ganzhou, Peoples R China
4.Wenshan Acad Agr Sci, Inst Upland Crops, Wenshan, Peoples R China
5.Yuxi Acad Agr Sci, Inst Crop Fertilizat, Yuxi, Peoples R China
6.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming, Yunnan, Peoples R China
7.Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Baoshan, Peoples R China
8.Chinese Acad Sci, Kunming Inst Bot, Ctr Mt Futures CMF, Kunming, Yunnan, Peoples R China
关键词： Citrus; Bacillus subtilis; endophyte; pathogen; restructuring; microbiome
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 12 卷
页码：
收录情况： SCI
摘要： Huanglongbing (HLB) has turned into a devastating botanical pandemic of citrus crops, caused by Candidatus Liberibacter asiaticus (CLas). However, until now the disease has remained incurable with very limited control strategies available. Restoration of the affected microbiomes in the diseased host through the introduction of an indigenous endophyte Bacillus subtilis L1-21 isolated from healthy citrus may provide an innovative approach for disease management. A novel half-leaf method was developed in vitro to test the efficacy of the endophyte L1-21 against CLas. Application of B. subtilis L1-21 at 10(4) colony forming unit (cfu ml(-1)) resulted in a 1,000-fold reduction in the CLas copies per gram of leaf midrib (10(7) to 10(4)) in 4 days. In HLB-affected citrus orchards over a period of 2 years, the CLas incidence was reduced to < 3%, and CLas copies declined from 10(9) to 10(4) g(-1) of diseased leaf midribs in the endophyte L1-21 treated trees. Reduction in disease incidence may corroborate a direct or an indirect biocontrol effect of the endophytes as red fluorescent protein-labeled B. subtilis L1-21 colonized and shared niche (phloem) with CLas. This is the first large-scale study for establishing a sustainable HLB control strategy through citrus endophytic microbiome restructuring using an indigenous endophyte.
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