Complete genome sequence of a new bipartite begomovirus infecting Boehmeria leiophylla in China
文献类型： 外文期刊
作者： Zhao, Liling 1 ; Zhong, Jing 1 ; Zhang, Xiaoyun 1 ; Ding, Ming 1 ; Zhang, Zhongkai 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Yunnan Key Lab Agr Biotechnol, 9 Xueyun Rd, Kunming 650223, Yunnan, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2018 年 163 卷 7 期
页码：
收录情况： SCI
摘要： A bipartite begomovirus was identified from a Boehmeria leiophylla plant sample exhibiting yellow mosaic symptoms collected in Nabanhe National Nature Reserve, Xishuangbanna, Yunnan, China. Five complete DNA-A and four DNA-B genome sequences were obtained by rolling-circle amplification (RCA), cloned, and sequenced. All DNA-A sequences were determined to be 2759 nucleotides in size, sharing 99.9%-100% nucleotide sequence identity with each other. The DNA-B sequences were comprised of 2673 nucleotides, sharing 98.6-100% nucleotide sequence identity with each other. Genomic organization of the begomovirus was typical of Old World bipartite begomoviruses. Sequence analysis revealed 81.84% nucleotide sequence identity to tomato leaf curl Hsinchu virus (ToLCHsV) from China for the DNA A component and 67.23% identity to the squash leaf curl China virus (SLCCNV) from India for the DNA B component. The sequence comparisons suggest that this bipartite begomovirus represents a novel species for which we propose the name "Ramie yellow mosaic virus".
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