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Functional characterization of the eugenol synthase gene (RcEGS1) in rose

文献类型： 外文期刊

作者： Yan, Huijun 1 ; Baudino, Sylvie 3 ; Caissard, Jean-Claude 3 ; Nicole, Florence 3 ; Zhang, Hao 2 ; Tang, Kaixue 2 ; Li, 1 ;

作者机构： 1.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Flower Res Inst, Kunming 650205, Yunnan, Peoples R China

3.Univ Lyon, CNRS, UJM St Etienne, BVpam FRE 3727, F-42023 St Etienne, France

关键词： Rose; Eugenol; RcEGS1; Protein expression; Functional analysis; VIGS

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2018 年 129 卷

页码：

收录情况： SCI

摘要： The floral volatile compound eugenol is an important constituent in many aromatic plants, being a floral attractant for pollinators as well as having antimicrobial activity. Rose flowers emit eugenol and its derivatives. We recently reported a eugenol synthase gene (RcEGS1) (JQ522949) that was present in petals of R. chinensis cv. Old Blush. RcEGS1 has its highest expression levels in the petals compared to other tissues; it has higher transcript levels at the developmental blooming stage and lower levels at budding and senescence stages. Here, we overexpressed the RcEGS1 protein in Escherichia coil, and showed by Western-blot analysis that its expression was mainly detected in stamens and petals at the flower opening stage. RcEGS1 was principally localized in the upper and lower epidermal layers, which are the major sites of scent emission in roses. Furthermore, we demonstrated that down-regulation of RcEGS1 expression in flowers by virus-induced gene silencing led to a reduction of the relative content of eugenol. We suggested that RcEGS1 was responsible for eugenol biosynthesis in roses.

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