Proteomic Analysis of the Secretory Proteins from Phytophthora infestans under Nitrogen Deficiency using Label-free LC-MS
文献类型： 外文期刊
作者： Yu, Ping 1 ; Dong, Chao 1 ; Yao, Chunxin 1 ; Ding, Yumei 1 ; Zhou, Xiaogang 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Key Lab Southwestern Crop Gene Resources & Germpl, Minist Agr, Kunming 650205, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Yunnan Prov Key Lab Agr Biotechnol, Biotechnol & Germplasm Resources Inst, Kunming 650205, Yunnan, Peoples R China
关键词： Effectors; Phytophthora infestans; Proteomics; Secretory proteins
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2018 年 20 卷 10 期
页码：
收录情况： SCI
摘要： Phytophthora infestans causes the late blight disease in potato, and its effectors have a potential role in its pathogenicity. Nitrogen is a key nutrient source affecting the growth and development of microbiota. Differentially expressed proteins secreted by the P. infestans strain NOD-1 were analyzed using label-free liquid chromatography-mass spectrometry quantitative proteomics in a complete medium or under nitrogen-deficient conditions to understand whether nitrogen stress promoted the secretion of more pathogenic proteins by P. infestans. A total of 5615 unique peptides and 1188 proteins were identified. Moreover, 93 differentially expressed proteins (containing signal peptides of Crinkling and necrosis-inducing protein and Arginine-X-leucine-arginine) and 28 specific proteins (localized extracellularly, in the cytoplasm, and in the plasma membrane) were detected under nitrogen-deficient conditions. Furthermore, 27 important effectors (mainly apoplastic and cytoplasmic), which had a potential role in the infection process, were screened. This study showed that the pathogenicity of P. infestans was enhanced under nitrogen-deficient conditions through the expression of effectors, particularly the cytoplasmic effectors. (C) 2018 Friends Science Publishers
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