文献类型： 外文期刊
作者： Guo, Rong 1 ; Guo, Hongyan 1 ; Zhang, Qingying 1 ; Guo, Mengbi 1 ; Xu, Yanping 1 ; Zeng, Min 2 ; Lv, Pin 1 ; Chen, Xuan 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Ind Crop Res Inst, Kunming, Yunnan, Peoples R China
2.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming, Yunnan, Peoples R China
关键词： Cannabis; wild; cultivated; reference gene; gene expression
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
ISSN： 0916-8451
年卷期： 2018 年 82 卷 11 期
页码：
收录情况： SCI
摘要： RT-qPCR has been widely used for gene expression analysis in recent years. The accuracy of this technique largely depends on the selection of suitable reference genes. In order to facilitate gene expression analysis in wild and cultivated Cannabis, the expression stability of seven candidate reference genes (ACT2, 18S rRNA, GAPDH, UBQ, TUB, PP2A and EF1 alpha) were assessed in leaves samples of different development stages and different organs of both wild and cultivated Cannabis in the present study. Their expression stabilities were evaluated through three software packages (GeNorm, Normfinder and Bestkeeper). Results showed that UBQ and EF1 alpha were the highly ranked genes in different leaves samples, and PP2A was the most stable reference gene in different organs, while GAPDH was the least stable one. And the validation of the reference genes selected was further confirmed by the expression patterns of MDS and OLS.
- 相关文献
作者其他论文 更多>>
-
The inclusion mode, characterization and cytotoxic activity evaluation of host-guest complex by β-cyclodextrin trimer with podophyllotoxin
作者：Gao, Haoqin;Qian, Rui;Yang, Jing;Yang, Rui;Li, Dan;Zhang, Jihong;Guo, Mengbi
关键词：Cyclodextrin; Podophyllotoxin; Inclusion complex; Molecular recognition
-
Interspecies/Intergroup Complementation of Orthotospovirus Replication and Movement through Reverse Genetics Systems
作者：Feng, Mingfeng;Chen, Minglong;Yuan, Yulong;Liu, Qinhai;Cheng, Ruixiang;Yang, Tongqing;Li, Luyao;Guo, Rong;Dong, Yongxin;Chen, Jing;Yang, Yawen;Yan, Yuling;Cui, Hongmin;Jing, Dong;Kang, Jinrui;Chen, Shuxian;Li, Jia;Zhu, Min;Tao, Xiaorong;Huang, Changjun;Zhang, Zhongkai;Kormelink, Richard
关键词：plant negative-stranded RNA viruses; reverse genetics system; orthotospovirus; replication; cell-to-cell movement
-
Cyclodextrin-based multifunctional supramolecular catalysts for aqueous CuAAC reaction
作者：Li, Jianbo;Feng, Xuepu;Wang, Wenting;Yang, Jing;Li, Xiang;Yang, Rui;Yang, Bo;Guo, Mengbi
关键词：Bioorthogonal chemistry; Click reaction; CuAAC; Supramolecular catalysis; Polycyclodextrin; Triazole
-
Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits
作者：Chen, Xuan;Guo, Hong-Yan;Zhang, Qing-Ying;Guo, Rong;Lv, Pin;Xu, Yan-Ping;Guo, Meng-Bi;Zhang, Yuan;Zhang, Kun;Yang, Ming;Wang, Lu;Zhan, Yi-Xun;Liu, Yan-Hu
关键词：Cannabis; Wild; Cultivated; Whole-genome resequencing; Genetic structure; Flowering
-
Preparation of poly(e-lysine)-cyclodextrin coated Fe3O4 nanoparticles for selective separation of natural medicine: Scutellarin
作者：Liao, Rongqiang;Jiang, Dexi;Liu, Ying;Lv, Pin;Lv, Pin
关键词：Cyclodextrin; Nanoparticles; Separation; Scutellarin
-
Highly efficient catalytic performance on CuAAC reaction by polymer-like supramolecular self-assemblies-Cu(I) in aqueous solution
作者：Deng, Bin;Yang, Jing;Yang, Rui;Guo, Mengbi
关键词：CuAAC reaction; cyclodextrin; green catalysis; supramolecular polymer
-
Acyclic cucurbit[n]urils-based supramolecular encapsulation for enhancing the protective effect of capsaicin on gastric mucosa and reducing irritation
作者：Zhou, Jiawei;Lv, Pin;Zhang, Shuqing;Zhang, Yazhou;Yang, Jing;Yang, Bo;Zhang, Ruihao;Lv, Pin
关键词：Acyclic cucurbit[n]urils; Capsaicin; Inclusion complex; Bioavailability; Gastric mucosal injury; Toxicity
