您好，欢迎访问云南省农业科学院 机构知识库！

Characterization of an acidic alpha-galactosidase from hemp (Cannabis sativa L.) seeds and its application in removal of raffinose family oligosaccharides (RFOs)

文献类型： 外文期刊

作者： Zhang, Weiwei 1 ; Du, Fang 1 ; Tian, Guoting 5 ; Zhao, Yongchang 5 ; Wang, Hexiang 3 ; Ng, Tzi Bun 6 ;

作者机构： 1.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

2.Peking Union Med Coll, Beijing 100193, Peoples R China

3.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

4.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China

5.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming 650223, Yunnan, Peoples R China

6.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

关键词： alpha-galactosidases; Cannabis sativa L.; purification; raffinose family oligosaccharides

期刊名称：ACTA BIOCHIMICA POLONICA （ 影响因子：2.149； 五年影响因子：2.175 ）

ISSN： 0001-527X

年卷期： 2018 年 65 卷 3 期

页码：

收录情况： SCI

摘要： An acidic alpha-galactosidase designated as hemp seed alpha-galactosidase (HSG) was purified from hemp (Cannabis sativa L.) seeds. By means of chromatographic procedures which involved chromatography on the cation-exchangers CM-cellulose and SP-Sepharose, chromatography on the anion-exchangers DEAE-cellulose and Q-Sepharose, and gel filtration on Superdex 75 using fast protein liquid chromatography, HSG was purified to electrophoretic homogeneity. Results of SDS-PAGE and gel filtration on FPLC Superdex 75 revealed that the enzyme was a monomeric protein with a molecular weight of 38 kDa. Sequences of the inner peptides of the alpha-galactosidase obtained by MALDI-TOF-MS showed that HSG was a novel alpha-galactosidase since there was a little similarity to the majority of alpha-galactosidases recorded in the literature. A pH of 3.0 and a temperature of 50 degrees C were optimal for the activity of the enzyme. The activity of HSG was inhibited by the chemical modification with N-bromosuccinimide (NBS) reagent. HSG contained 16 tryptophan residues and two tryptophan residues on the surface, which were crucial to the alpha-galactosidase activity. The heavy metal ions Cd2+, Cu2+, Hg2+ and Zn2+ inhibited its activity. The K-m and V-max for the hydrolysis of pNPGal (4-nitrophenyl alpha-D-galactopyranoside) were respectively 0.008 mM and 68 mu M min(-1) mg(-1). HSG also catalyzed the hydrolysis of raffinose and other natural substrates. Hence the alpha-galactosidase possesses a tremendous potential for food and feed industries in the elimination of indigestible oligosaccharides from leguminous products.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台