文献类型： 外文期刊

作者： Shan, Liuying ¹ ; Haseeb, Hafiz Abdul ¹ ; Zhang, Jun ¹ ; Zhang, Dandan ¹ ; Jeffers, Daniel Paul ² ; Dai, Xiaofeng ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Agroprod Qual & Safety Control Storage &, Inst Food Sci & Technol, Beijing 100193, Peoples R China

2.Yunnan Acad Agr Sci, Inst Food Crops, CIMMYT Yunnan Off, 2238 Beijing Rd, Ku

关键词： Fusarium temperatum; Rapid diagnosis; LAMP; 28S rDNA; Maize

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.277； 五年影响因子：5.388 ）

ISSN： 0168-1605

年卷期： 2019 年 291 卷

页码：

收录情况： SCI

摘要： Fusarium temperatum is an emerging maize pathogen that causes maize ear and stalk rot diseases and produces various mycotoxins including moniliformin, beauvericin, enniatins and fumonisin B1, which poses a potential risk to the human food or animal feed supply chains. Early detection of F. temperatum is crucial to prevent its derived mycotoxins from entering the food chain, and is also a useful tool in disease management practices. Here, we describe a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of F. temperatum. The 28S ribosomal DNA sequences (28S rDNA) of F. temperatum were used to design a set of six primers. The reaction conditions were optimized for developing a fast assay with high specificity and sensitivity, and were able to detect the presence of less than 10 pg of target DNA per reaction within 60 min. Furthermore, the resulting amplicons were visualized by adding SYBR Green I to the reaction tubes. Suspected F. temperatum infected maize stalk samples collected from Yunnan province, China were identified using the developed LAMP assay. In conclusion, the method not only provides a rapid and specific screening for the existence of F. temperatum in a bulk of maize samples without using sophisticated equipment, but also is potentially useful for other agriculturally important toxigenic fungi,