您好，欢迎访问云南省农业科学院 机构知识库！

Alternative splicing coupled to nonsense-mediated mRNA decay contributes to the high-altitude adaptation of maca (Lepidium meyenii)

文献类型： 外文期刊

作者： Shi, Yong 1 ; Su, Zechun 2 ; Yang, Hong 1 ; Wang, Wenzhi 1 ; Jin, Guihua 1 ; He, Guiqing 2 ; Siddique, Abu Nasar 1 ; Zhan 1 ;

作者机构： 1.Chinese Acad Sci, Germplasm Bank Wild Species Southwest China, Kunming Inst Bot, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Alpine Econ Plant Res Inst, Lijiang 674100, Yunnan, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Southwest Forestry Univ, Sch Life Sci, Kunming 650224, Yunnan, Peoples R China

5.Bacha Khan Univ, Dept Biotechnol, Charsadda 24420, Pakistan

6.Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Fujian, Peoples R China

关键词： Alpine plants; Post-transcriptome; Intron retention; Alternative 3 ' splice site; Long non-coding RNA; Positive selection

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2019 年 694 卷

页码：

收录情况： SCI

摘要： Alpine plants remain the least studied plant communities in terrestrial ecosystems. However, how they adapt to high-altitude environments is far from clear. Here, we used RNA-seq to investigate a typical alpine plant mace (Lepidium meyenii) to understand its high-altitude adaptation at transcriptional and post-transcriptional level. At transcriptional level, we found that maca root significantly up-regulated plant immunity genes in day-time comparing to night-time, and up-regulated abiotic (cold/osmotic) stress response genes in Nov and Dec comparing to Oct. In addition, 17 positively selected genes were identified, which could be involved in mitochondrion. At post-transcriptional level, we found that maca had species-specific characterized alternative splicing (AS) profile which could be influenced by stress environments. For example, the alternative 3' splice site events (A3SS, 39.62%) were predominate AS events in maca, rather than intron retention (IR, 23.17%). Interestingly, besides serine/arginine-rich (SR) proteins and long non-coding RNAs (lncRNAs), a lot of components in nonsense-mediated mRNA decay (NMD) were identified under differential alternative splicing (DAS), supporting AS coupled to NMD as essential mechanisms for maca's stress responses and high-altitude adaptation. Taken together, we first attempted to unveil maca's high-altitude adaptation mechanisms based on transcriptome and post-transcriptome evidence. Our data provided valuable insights to understand the high-altitude adaptation of alpine plants.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台