Density enhancement of a faba bean genetic linkage map (Vicia faba) based on simple sequence repeats markers
文献类型： 外文期刊
作者： Yang, Tao 1 ; Jiang, Junye 1 ; Zhang, Hongyan 1 ; Liu, Rong 1 ; Strelkov, Stephen 2 ; Hwang, Sheau-Fang 3 ; Chang, Kan 1 ;
作者机构： 1.Natl Key Facil Crop Gene Resources & Genet Improv, Inst Crop Sci, Beijing, Peoples R China
2.Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada
3.Alberta Dept Agr, Crop Diversificat Ctr North, Edmonton, AB, Canada
4.Yunnan Acad Agr Sci, Inst Grain Crops, Kunming, Yunnan, Peoples R China
5.Agr Inst Riparian Reg, Yangzhou, Jiangsu, Peoples R China
关键词： density enhancement; faba bean; genetic linkage map; SSR markers
期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）
ISSN： 0179-9541
年卷期： 2019 年 138 卷 2 期
页码：
收录情况： SCI
摘要： Genetic mapping for faba bean lags far behind other major crops. Density enhancement of the faba bean genetic linkage map was carried out by screening 5,325 genomic SSR primers and 2033 expressed sequence tag (EST)-SSR primers on the parental cultivars '91825' and 'K1563'. Two hundred and fifteen genomic SSR and 133 EST-SSR primer pairs that detected polymorphisms in the parents were used to screen 129 F-2 individuals. This study added 337 more SSR markers and extended the previous linkage map by 2928.45cM to a total of 4516.75cM. The number of SSR markers in the linkage groups varied from 12 to 136 while the length of each linkage group ranged from 129.35 to 1180.21cM. The average distance between adjacent loci in the enhanced genetic linkage map was 9.71cM, which is 2.79cM shorter than the first linkage map of faba bean. The density-enhanced genetic map of faba bean will be useful for marker-assisted selection and breeding in this important legume crop.
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