文献类型： 外文期刊

作者： Kong, C. S. ¹ ; Chen, J. H. ¹ ; Liu, J. H. ² ; Yu, L. ³ ;

作者机构： 1.Yunnan Acad Agr Sci, Inst Agr Environm & Resources, Kunming 650205, Yunnan, Peoples R China

2.Yunnan Tobacco Ind Co Ltd, Technol Ctr, Kunming 650231, Yunnan, Peoples R China

3.Kunming Univ, Yunnan Urban Agr Engn & Technol Res Ctr, Kunming 650214, Yunnan, Peoples R China

关键词： tobacco; molecular cloning; cadmium resistance protein 2; metal tolerance protein 4; tissue expression

期刊名称：CYTOLOGY AND GENETICS （ 影响因子：0.579； 五年影响因子：0.576 ）

ISSN： 0095-4527

年卷期： 2019 年 53 卷 2 期

页码：

收录情况： SCI

摘要： The complete coding sequences of common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes were amplified by RT-PCR. The tobacco cadmium resistance protein 2 gene encodes a protein of 192 amino acids which shares high identity with the cadmium resistance protein 2 of four speciesnicotiana tomentosiformis (99%), wood tobacco (97%), capsicum annuum (90%) and potato (86%). The tobacco metal tolerance protein 4 gene encodes a protein of 415 amino acids which shares high identity with the metal tolerance protein 4 of six speciesnicotiana tomentosiformis (90%), wood tobacco (90%), lycopersicon esculentum (84%), potato (84%), lycopersicon pennellii (83%) and capsicum annuum (82%). Prediction of transmembrane helices showed that cadmium resistance protein 2 and metal tolerance protein 4 might be two transmembrane proteins. Phylogenetic analysis revealed that the common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes both have a closer genetic relationship with the wood tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes. Computer-assisted analysis showed that cadmium resistance protein 2 gene is structured in 4 exons and 3 introns and metal tolerance protein 4 gene consists of 7 exons and 6 introns. The gene expression profile analysis indicated that the common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes were highly expressed in root.