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ESTABLISHMENT OF EFFICIENT ADVENTITIOUS SHOOTS INDUCTION SYSTEM AND EX VITRO ROOTING IN VACCINIUM CORYMBOSUM (ERICACEAE)

文献类型： 外文期刊

作者： Wang, Ye 1 ; Dong, Xian 1 ; Huang, Heng-Yu 1 ; Wang, Yuan-Zhong 2 ;

作者机构： 1.Yunnan Univ Chinese Med, Yunnan Breeding & Cultivat Res & Dev Ctr Endanger, Kunming, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Inst Med Plants, Kunming, Yunnan, Peoples R China

关键词： Adventitious shoot; blueberry; callus; orthogonal experiments; regeneration; rooting

期刊名称：BOTANICAL SCIENCES （ 影响因子：0.959； 五年影响因子：1.076 ）

ISSN： 2007-4298

年卷期： 2019 年 97 卷 2 期

页码：

收录情况： SCI

摘要： Background: The extension of the blueberry industry in China is restricted by the low performance of proliferation coefficient, transplanting survival rate and the long cycle production. Hypothesis: We hypothesized the suitable medium with optimal concentration and type combination would improve the survival rate of Vaccinium corymbosum. Species studied: Vaccinium corymbosum L. cultivar 'O'Neal. Study site and years of study: Our study was conducted in Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese medicinal materials, Yunnan University of Chinese Medicine since November 2015. Methods: Efficient protocol of plant regeneration about Southern highbush blueberry (cultivar 'O'Neal) was established using annual shoots via single factor and orthogonal experiments. Results: Olive medium with 2.0 mg.L-1 zeatin was found to be most suitable for initiation culture. The highest callus induction and bud formation rate were determined with 93.67 % and 95.57 %, respectively. Furthermore, as the germination of axillary buds, numerous shoots were formed with the proliferation coefficient (> 60). Well-developed shoots were obtained using rejuvenation culture on half-strength Olive medium supplemented with combination of 2.0 mg.L-1 indole-3-butyric acid, 1.0 mg.L-1 naphthaleneacetic acid, 0.01 mg.L-1 chlormequat chloride and 0.5 g.L-1 activated charcoal. The rooting rate could reach to 100 % using 2 cm length of well-developed shoots transferred into the 5:1 sterilized peat: perlite, which was enhanced from 27.61 up to 95 % after transplanting to the field. Conclusions: An efficient system for plant regeneration from bud induction to transplanting to the field was established to maintain the characteristics of southern highbush blueberry varieties.

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