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Traceability the provenience of cultivated Paris polyphylla Smith var. yunnanensis using ATR-FTIR spectroscopy combined with chemometrics

文献类型： 外文期刊

作者： Wu, Xue-Mei 1 ; Zhang, Qing-Zhi 2 ; Wang, Yuan-Zhong 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Yunnan, Peoples R China

2.Yunnan Univ Tradit Chinese Med, Coll Tradit Chinese Med, Kunming 650500, Yunnan, Peoples R China

关键词： Paris polyphylla Smith var. yunnanensis; Data fusion; ATR-FT1R; PIS-DA; Random forest; Biomass

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）

ISSN： 1386-1425

年卷期： 2019 年 212 卷

页码：

收录情况： SCI

摘要： The conventional procedures, based on attenuated total reflectance-Fourier transform infrared spectrometry (ATR-FTIR), have been developed for the origins traceability of cultivated Paris polyphylla Smith var. yunnanensis (PPY) samples with the help of partial least square discriminant analysis (PLS-DA) and random forest. In this study, a set of 219 batch cultivated PPY samples, containing the cultivation years of 5, 6 and 7, and covering the municipal districts of Chuxiong, Dali, Honghe, Lijiang and Yuxi in Yunnan Province, China, were used to build the discrimination models. Firstly, a visualized analysis was carried out by t-distributed stochastic neighbor embedding (t-SNE) to reduce each data point in a two-dimensional map and make a knowledge of the sample distribution tendency. Secondly, the single spectra data sets of Paridis rhizome and leaf tissues, and the combination of these two data sets with variable selection (mid-level data fusion strategy), were used to establish PLS-DA and random forest models, and parallelly compared the model performance. Results demonstrated that the discrimination ability of PLS-DA preceded the random forest model, and the classification performance was remarkably improved after mid-level data fusion. These results verified each other by 5-, 6- and 7-year old Paridis samples and indicated that the model performance established in the present study was reliable. Besides, five agronomic characters, including the plant height, dry weight of rhizome and leaf tissues, and the allocation of rhizome and leaf were determined and analyzed, results of which indicated that the dry weight and their allocation was significantly different among various origins and fluctuated with the cultivation years. This study was using a comprehensive and green analytical method to discriminate the cultivated Paridis according to their provenances, which was simultaneously benefited for the appropriate cultivation areas selection based on the dry weight of rhizome tissues. (C) 2019 Elsevier B.V. All rights reserved.

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