Identification of differentially expressed genes in fragrant rose Jinyindao with suppressive subtraction hybridization
文献类型： 外文期刊
作者： Xie Jirong 2 ; Xiong Yunhai 2 ; Liang Guolu 1 ; Cheng Zaiquan 3 ; Tang Kaixue 3 ; Huang Xingqi 3 ;
作者机构： 1.SW Univ, Coll Hort & Gardens, Chongqing 400715, Peoples R China
2.Chongqing Univ Arts & Sci, Dept Life Sci, Chongqing 402168, Peoples R China
3.Yunnan Acad Agr Sci, Kunming 650223, Peoples R China
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
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收录情况： SCI
摘要： To investigate the floral fragrance new genes, scent mutant of rose was used here. The suppressive subtraction hybridization (SSH) technique and micoarray analysis of the clones were used to isolate the cDNA fragments, which showed differential expression between the rose scent mutant 'Wangriqinghuai' and wild type 'Jinyindao' (Rosa x hybrida), and RT-PCR was used to identify up-regulated expressed genes. 16 positive contigs of JSSH were obtained. Some ESTs such as RcOMT1, RcOMT2, RhMYB92 and RhGP1 were known to regulate scent metabolism, and 5 ESTs with no homology in NCBI may represent new genes involved in rose flower fragrance metabolism. SSH technique combined with cDNA micoarray would be useful for analysis and isolation of the genes related to rose floral scent.
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