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Development and application of sugarcane streak mosaic virus vectors

文献类型： 外文期刊

作者： Yin, Yuteng 1 ; Wang, Duan 1 ; Wang, Hongwei 1 ; Sun, Yanwei 1 ; Yin, Cece 1 ; Li, Jie 3 ; Ye, Jian 1 ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China

3.Yunnan Acad Agr Sci, Sugarcane Res Inst, Yunnan Key Lab Sugarcane Genet Improvement, Kaiyuan 661699, Peoples R China

关键词： Sugarcane streak mosaic virus; Sugarcane; Reverse genetics; Infectious cDNA clone; Expression vector

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2024 年 593 卷

页码：

收录情况： SCI

摘要： Sugarcane streak mosaic virus (SCSMV) is one of the major pathogens of sugarcane in the world. Molecular studies and disease management of SCSMV are hindered by the lack of efficient infectious clones. In this study, we successfully constructed Agrobacterium infiltration based infectious clone of SCSMV with different variants. Infectious clones of wild type SCSMV could efficiently infect Nicotiana benthamiana and sugarcane plants resulting in streak and mosaic symptoms on systemic leaves which were further confirmed with RT-PCR and serological assays. SCSMV variants of less adenylation displayed attenuated pathogenicity on N.benthamiana. SCSMV-based recombinant heterologous EGFP protein vector was also developed. The EGFP-tagged recombinant SCSMV could highly expressed in vegetative organs including roots. These infectious clones of SCSMV could be further developed for platform tools for both biotechnological studies and management of SCSMV disease.

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