Integrative Analysis of miRNAs and Their Targets Involved in Ray Floret Growth in Gerbera hybrida
文献类型： 外文期刊
作者： Chen, Yanbo 1 ; Liao, Bingbing 1 ; Lin, Xiaohui 1 ; Luo, Qishan 1 ; Huang, Xuanyan 1 ; Wang, Xiaojing 1 ; Shan, Qinli 3 ; Wang, Yaqin 1 ;
作者机构： 1.South China Normal Univ, Guangdong Prov Key Lab Biotechnol Plant Dev, Sch Life Sci, Guangzhou 510631, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
3.Yunnan Acad Agr Sci, Flower Res Inst, Natl Engn Res Ctr Ornamental Hort, Kunming 650205, Yunnan, Peoples R China
关键词： miRNA; petal growth and development; anthocyanin; Gerbera hybrida
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 13 期
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) are involved in regulating many aspects of plant growth and development at the post-transcriptional level. Gerbera (Gerbera hybrida) is an important ornamental crop. However, the role of miRNAs in the growth and development of gerbera is still unclear. In this study, we used high-throughput sequencing to analyze the expression profiles of miRNAs in ray floret during inflorescence opening. A total of 164 miRNAs were obtained, comprising 24 conserved miRNAs and 140 novel miRNAs. Ten conserved and 15 novel miRNAs were differentially expressed during ray floret growth, and 607 differentially expressed target genes of these differentially expressed miRNAs were identified using psRNATarget. We performed a comprehensive analysis of the expression profiles of the miRNAs and their targets. The changes in expression of five miRNAs (ghy-miR156, ghy-miR164, ghy-miRn24, ghy-miRn75 and ghy-miRn133) were inversely correlated with the changes in expression of their eight target genes. The miRNA cleavage sites in candidate target gene mRNAs were determined using 5 '-RLM-RACE. Several miRNA-mRNA pairs were predicted to regulate ray floret growth and anthocyanin biosynthesis. In conclusion, the results of small RNA sequencing provide valuable information to reveal the mechanisms of miRNA-mediated ray floret growth and anthocyanin accumulation in gerbera.
- 相关文献
作者其他论文 更多>>
-
Quantification of plasmodesmata frequency under three-dimensional view using focused ion beam-scanning electron microscopy and image analysis
作者：Deng, Yinlu;Wang, Yaqin;Hong, Jian;Li, Zhenghe;Xie, Li;Deng, Yinlu;Li, Zhenghe;Shang, Weina;Shang, Weina;Zhang, Xiaomin;Hong, Jian;Xie, Li;Guo, Jiansheng;Guo, Jiansheng;Zhang, Zhongkai
关键词：PD quantification; PD frequency; Focused ion beam-scanning electron micro; scopy; Image analysis
-
Unraveling the complex evolutionary history of lepidopteran chromosomes through ancestral chromosome reconstruction and novel chromosome nomenclature
作者：Chen, Xi;Wang, Zuoqi;Zhang, Chaowei;Hu, Jingheng;Lu, Yueqi;Zhou, Hang;Mei, Yang;Cong, Yuyang;Guo, Fangyuan;He, Kang;Li, Fei;Chen, Xi;Wang, Zuoqi;Zhang, Chaowei;Hu, Jingheng;Lu, Yueqi;Zhou, Hang;Mei, Yang;Cong, Yuyang;Guo, Fangyuan;He, Kang;Li, Fei;Wang, Yaqin;Liu, Ying
关键词：Lepidoptera; Sex chromosome; Comparative genomics; Sexual dimorphism; Genome evolution
-
In vitro regeneration of the colorful fern Pteris aspericaulis var. tricolor via green globular bodies system
作者：Yu, Rongpei;Li, Fan;Wang, Guoxian;Ruan, Jiwei;Wu, Lifang;Wu, Min;Yang, Chunmei;Shan, Qinli;Yu, Rongpei;Yu, Rongpei;Li, Fan;Wang, Guoxian;Ruan, Jiwei;Wu, Lifang;Shan, Qinli
关键词：Pteris aspericaulis var; tricolor; In vitro regeneration; Green globular bodies; LEDs
-
Haploid induction via unpollinated ovule culture in Gerbera hybrida
作者：Li, Fan;Yu, Rongpei;Li, Huimin;Wang, Lihua;Li, Shenchong;Shan, Qinli;Cheng, Ying;Zhao, Xiaokun
关键词：
-
The Effect of Temperature on Plant Growth in Four Gerbera hybrida Cultivars
作者：Li, Fan;Li, Shenchong;Shan, Qinli
关键词：temperature response; plant height; biomass accumulation; leaf area; photosynthesis
-
Effects of Seasonal Variation and Gibberellic Acid Treatment on the Growth and Development of Gypsophila paniculata
作者：Li, Fan;Wang, Guoxian;Yu, Rongpei;Wu, Min;Shan, Qinli;Wu, Lifang;Ruan, Jiwei;Yang, Chunmei
关键词：
-
Weed-suppression ability of Oryza longistaminata and Oryza sativa
作者：Zhanq, Fudou;Li, Tianlin;Shan, Qinli;Guo, Yiqing;Xu, Peng;Hu, Fengyi;Tao, Dayun
关键词：allelopathy;competition;Oryza Longistaminata;weed management
