Homogenization-circulating ultrasound in combination with aqueous enzymatic pretreatment for microwave-assisted extraction of kernel oil and essential oil from the fruit of Litsea cubeba
文献类型： 外文期刊
作者： Yang, Xinyu 1 ; Yang, Yang 1 ; Zhang, Ke 1 ; Zhao, Ru 2 ; Tian, Hao 3 ; Yang, Lei 1 ; Zhao, Xiuhua 1 ;
作者机构： 1.Northeast Forestry Univ, Coll Chem Chem Engn & Resource Utilizat, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China
2.Nanjing Xiaozhuang Univ, Key Lab Qual & Safety Agr Prod Nanjing, Nanjing 211171, Peoples R China
3.Yunnan Acad Agr Sci, Agroprod Proc Res Inst, Kunming 650223, Peoples R China
关键词： Homogenization-circulating ultrasound in; combination with aqueous enzymatic; pretreatment (HCUEP); Litsea cubeba; Seed oil; Essential oil; Microwave-assisted extraction
期刊名称：ULTRASONICS SONOCHEMISTRY （ 影响因子：9.7； 五年影响因子：9.1 ）
ISSN： 1350-4177
年卷期： 2024 年 111 卷
页码：
收录情况： SCI
摘要： Homogenization-circulating ultrasound in combination with an aqueous enzymatic pretreatment microwaveassisted extraction (HCUEPM) technique was successfully applied to extract kernel oil and essential oil from Litsea cubeba fruits. After screening the types and concentrations of enzymes, a 3 % pectinase aqueous solution was chosen. The Plackett-Burman design was used to screen eight parameters that might affect the yield of kernel oil and essential oil to identify significant variables. The best conditions were then predicted by further optimizing statistically significant factors via the Box-Behnken design. The optimal conditions were as follows: stirring speed of 1000 r/min, environmental pH of 5, homogenization time of 4 min, duty cycle of 20 %, ultrasound irradiation power of 400 W, incubation temperature of 52.78 degrees C, liquid-solid ratio of 9.31 mL/g, and incubation time of 2.53 h. Three parallel experiments were conducted under these conditions, yielding actual kernel oil at 240.56 +/- 11.07 mL/kg DW and essential oil at 64.89 +/- 3.1 mL/kg DW, which are close to the theoretical values. Compared with the HCUEPM method, the homogenization-microwave-assisted hydrodistillation (HMHD) method yielded 65.63 +/- 3.2 mL/kg DW of essential oil but could not extract kernel oil. These findings demonstrate that the HCUEPM used in this study can efficiently extract a significant amount of kernel oil and essential oil from L. cubeba fruits in a short period of time. GC-MS analysis of the kernel oil and essential oil extracted via different methods revealed no significant differences in composition. The main components of the essential oil were D-limonene, trans-citral, cis-citral, and citronellal. The main components of the kernel oil were C10 and C12 medium-chain fatty acids, laying the foundation for the potential application of L. cubeba kernel oil and essential oil in the field of human health.
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