文献类型： 外文期刊

作者： Li, F. ¹ ; Cheng, Y. ² ; Yu, R. ¹ ; Yang, C. ¹ ;

作者机构： 1.Yunnan Acad Agr Sci, Natl Engn Res Ctr Ornamental Hort, Floriculture Res Inst, Key Lab Flower Breeding Yunnan Prov, Kunming, Yunnan, Peoples R China

2.Yunnan Univ, Sch Agr, Kunming, Yunnan, Peoples R China

关键词： Genomics; Germplasm; Lisianthus; Nuclear DNA amount; Polyploidy

期刊名称：JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY （ 影响因子：1.279； 五年影响因子：1.462 ）

ISSN： 1680-7073

年卷期： 2022 年 24 卷 3 期

页码：

收录情况： SCI

摘要： Eustoma grandiflorum is considered as the `next rose' in floriculture, ranking top-ten in cut flowers. In this study, we investigated the ploidy level and genome size of 28 commercial cultivars of E. grandiflorum through flow cytometry. The analysis of each cultivar showed that only one cultivar was tetraploid, whereas the rest were diploids. By comparing with a standard reference genome of Solanum lycopersicum, the genome size (1C) of E. grandiflorum cultivars ranged from 1.26 to 2.64 Gb, which was in line with their ploidy levels and previous data. Although a large number of plant phenotypic diversities were observed in the experimental cultivars, the genome size displayed little difference in diploids, indicating that the monoploid DNA amount of E. grandiflorum is relatively conserved during artificial selection. It is possible to sequence the genome of E. grandiflorum using the latest sequencing techniques, which could provide a solid foundation for molecular biology research and molecular breeding for E. grandiflorum.