文献类型： 外文期刊
作者： Chen, Qin 1 ; Lei, Jun 1 ; Li, Xiaolei 1 ; Zhang, Jinyu 1 ; Liu, Diqiu 1 ; Cui, Xiuming 1 ; Ge, Feng 1 ;
作者机构： 1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Key Lab Sustainable Utilizat Panax Notoginseng Res, Kunming 650500, Peoples R China
2.Kunming Univ Sci & Technol, Analyt & Testing Res Ctr, Kunming 650500, Peoples R China
3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650205, Peoples R China
4.State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China
关键词： Panax notoginseng; Synthetic biology; Metabolic engineering; Saponin
期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.1 ）
ISSN： 0176-1617
年卷期： 2024 年 299 卷
页码：
收录情况： SCI
摘要： Ginsenoside F1 has high medicinal values, which is a kind of rare triterpene saponin isolated from Panax plants. The extremely low content of ginsenoside F1 in herbs has limited its research and application in medical field. In this work, we constructed a pathway in tobacco for the biosynthesis of ginsenoside F1 by metabolic engineering. Four enzyme genes ( PnDDS , CYP716A47, CYP716S1 and UGT71A56 ) isolated from Panax notoginseng were introduced into tobacco. Thus, a biosynthetic pathway for ginsenoside F1 synthesis was artificially constructed in tobacco cells; moreover, the four exogenous genes could be expressed in the roots, stems and leaves of transgenic plants. Consequently, ginsenoside F1 and its precursors were successfully synthesized in the transgenic tobacco, compared with Panax plants, the content of ginsenoside F1 in transgenic tobacco was doubled. In addition, accumulation of ginsenoside F1 and its precursors in transgenic tobacco shows organ specificity. Based on these results, a new approach was established to produce rare ginsenoside F1; meanwhile, such strategy could also be employed in plant hosts for the heterologous synthesis of other important or rare natural products.
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