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Three-dimensional analysis of membrane structures associated with tomato spotted wilt virus infection

文献类型： 外文期刊

作者： Guo, Jiansheng 1 ; Wang, Guan 2 ; Xie, Li 3 ; Wang, Xinqiu 3 ; Feng, Lingchong 2 ; Guo, Wangbiao 2 ; Tao, Xiaorong 4 ; Humbel, Bruno M. M. 2 ; Zhang, Zhongkai 6 ; Hong, Jian 3 ;

作者机构： 1.Zhejiang Univ, Dept Pathol, Sir Run Run Shaw Hosp, Sch Med, Hangzhou, Peoples R China

2.Zhejiang Univ, Ctr Cryoelectron Microscopy, Sch Med, Hangzhou 310058, Peoples R China

3.Zhejiang Univ, Ctr Anal & Measurement, Hangzhou 310029, Peoples R China

4.Nanjing Agr Univ, Dept Plant Pathol, Nanjing, Peoples R China

5.Okinawa Inst Sci & Technol OIST, Imaging, Onna, Okinawa, Japan

6.Yunnan Acad Agr Sci, Inst Biotechnol & Genet Resources, Yunnan Prov Key Lab Agribiotechnol, Kunming 650223, Peoples R China

关键词： 3D reconstruction in large volume; focused ion beam scanning electron microscopy; viral morphogenesis; viral trafficking

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.947； 五年影响因子：8.452 ）

ISSN： 0140-7791

年卷期：

页码：

收录情况： SCI

摘要： To study viral infection, the direct structural visualization of the viral life cycle consisting of virus attachment, entry, replication, assembly and transport is essential. Although conventional electron microscopy (EM) has been extremely helpful in the investigation of virus-host cell interactions, three-dimensional (3D) EM not only provides important information at the nanometer resolution, but can also create 3D maps of large volumes, even entire virus-infected cells. Here, we determined the ultrastructural details of tomato spotted wilt virus (TSWV)-infected plant cells using focused ion beam scanning EM (FIB-SEM). The viral morphogenesis and dynamic transformation of paired parallel membranes (PPMs) were analyzed. The endoplasmic reticulum (ER) membrane network consisting of tubules and sheets was related to viral intracellular trafficking and virion storage. Abundant lipid-like bodies, clustering mitochondria, cell membrane tubules, and myelin-like bodies were likely associated with viral infection. Additionally, connecting structures between neighboring cells were found only in infected plant tissues and showed the characteristics of tubular structure. These novel connections that formed continuously in the cell wall or were wrapped by the cell membranes of neighboring cells appeared frequently in the large-scale 3D model, suggesting additional strategies for viral trafficking that were difficult to distinguish using conventional EM.

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