Evaluation of a phenolic-tolerant Wickerhamomyces anomalus strain for efficient ethanol production based on omics analysis
文献类型： 外文期刊
作者： Qin, Shiwen 1 ; Hu, Faguang 2 ; Kong, Deting 1 ; Zhao, Tonghua 3 ; Suo, Yukai 3 ; He, Feifei 1 ;
作者机构： 1.Yunnan Univ, Sch Agr, Kunming 650500, Peoples R China
2.Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Baoshan 678000, Peoples R China
3.Yunnan Minzu Univ, Key Lab Nat Prod Synthet Biol Ethn Med Endophytes, State Ethn Affairs Commiss, Kunming 650504, Peoples R China
4.Yunnan Minzu Univ, Key Lab Chem Ethn Med Resources, State Ethn Affairs Commiss, Kunming 650031, Peoples R China
5.Yunnan Minzu Univ, Minist Educ, Kunming 650031, Peoples R China
关键词： Bioethanol; Wickerhamomyces anomalus; Phenolic resistance; Omics analysis; Coffee husk
期刊名称：ENERGY （ 影响因子：9.4； 五年影响因子：8.8 ）
ISSN： 0360-5442
年卷期： 2024 年 311 卷
页码：
收录情况： SCI
摘要： Lignocellulosic biomass is a highly economically viable substrate for ethanol fermentation, but the presence of phenolic compounds (PCs) in lignocellulosic hydrolysates severely hinders the growth of ethanologenic strains. This study aims to investigate the ethanol fermentation capabilities and PC tolerance of the newly isolated Wickerhamomyces anomalus CAP5. The strain demonstrated remarkable ethanol production, achieving 87.1 g/L with a yield of 0.47 g/g, exceeding 90 % of the theoretical maximum. To elucidate the mechanisms responsible for this high ethanol yield, comprehensive genome and transcriptome analyses were performed. These analyses identified key metabolic pathways, including acetate reabsorption and an incomplete glycerol synthesis pathway, as crucial factors contributing to the strain's superior performance. Moreover, W. anomalus CAP5 demonstrated notable resistance to PCs, particularly phenolic acids. In fermenting with coffee husk hydrolysate (CHH), which is rich in PCs, W. anomalus CAP5 achieved noteworthy ethanol production of 60.5 g/L, marking a record for ethanol production from CHH. Hence, W. anomalus CAP5 emerges as a promising strain for effective ethanol production from lignocellulosic hydrolysates enriched with PCs.
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