文献类型： 外文期刊

作者： Wang, M. Q. ¹ ; Xu, D. L. ³ ; Li, R. ³ ; Zhou, G. H. ¹ ;

作者机构： 1.S China Agr Univ, Lab Plant Virol, Guangzhou 510642, Guangdong, Peoples R China

2.Yunnan Acad Agr Sci, Sugarcane Res Inst, Key Lab Sugarcane Genet Improvement Yunnan Prov, Kaiyuan 661600, Peoples R China

3.ARS, USDA, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA

关键词： genotypic diversity;geographical distribution;RT-PCR-RFLP;Saccbarum;Sugarcane yellow leaf virus

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

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收录情况： SCI

摘要： A survey of Sugarcane yellow leaf virus (SCYLV) in China showed infection rates of 1·1-42·4% in six different sugarcane growing provinces, with an average rate of 20-3% (129/634), and no infection in one province. The majority of the infected plants showed yellowing of the midrib on the abaxial surface of leaves. Genotypic diversity of the 129 SCYLV isolates was analysed by a reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) assay and nucleotide sequencing. Sixty-six isolates from Guangxi, Yunnan, Hainan, Fujian and Guizhou provinces were of the genotype BRA, whereas another 63 isolates from Guangdong province consisted of five genotypes, BRA, CHN1, CUB, PER and a novel genotype, CHN2. Among these genotypes, BRA was the predominant one identified in 43 samples (68·3%). Mixed infection of BRA and PER was also found in two samples. Phylogenetic analysis based on a 1·3-kb nucleotide sequence (including ORFs 3-4 and partial ORF 5) supported the identification of the novel CHN2 genotype. CHN2 shared 91·9-97·8% identity with five other known genotypes and was most closely related to the CHN1 genotype (97·6-97·8% identities). The nearly complete genomic sequences of seven isolates representing genotypes BRA, CUB and PER were determined, and they shared nucleotide sequence identities of 97·2-97·5%, 98·4—98·9% and 98·4%, respectively, with previously reported sequences of the corresponding genotypes.