文献类型: 外文期刊
作者: Li, Wen-Feng 1 ; Wang, Xiao-Yan 1 ; Huang, Ying-Kun 1 ; Shen, Ke 1 ; Shan, Hong-Li 1 ; Luo, Zhi-Ming 1 ; Yin, Jiong 1 ; J 1 ;
作者机构: 1.Yunnan Acad Agr Sci, Yunnan Key Lab Sugarcane Genet Improvement, Sugarcane Res Inst, Kaiyuan 661600, Peoples R China
2.Baoshan Sugarcane Res Inst, Baoshan 678000, Peoples R China
3.Baoshan Sugarcane R
关键词: Phytoplasma;PCR detection;sugarcane white leaf
期刊名称:CANADIAN JOURNAL OF PLANT PATHOLOGY ( 影响因子:2.442; 五年影响因子:1.993 )
ISSN:
年卷期:
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收录情况: SCI
摘要: The introduction of the sugarcane white leaf (SCWL) phytoplasma into Yunnan Province, China was confirmed for the first time by nested PCR. Sequencing and analysis of 17 nested PCR products of the 16S rDNA of the phytoplasma from random samples showedthat these sequences were identical. A BLAST search indicated that the sequences were highly homologous (99.05-100% similarity) to the corresponding genome region in GenBank. Field surveys showed that disease incidence among affected cultivars was 13-52% and up to 100% in seriously infected fields. The sugarcane cultivar 'Yuetang86-368' was highly susceptible. SCWL is a destructive disease on sugarcane that requires preventive quarantine management measures to prevent its further spread.
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