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Nucleocapsid of Tomato spotted wilt tospovirus forms mobile particles that traffic on an actin/endoplasmic reticulum network driven by myosin XI-K

文献类型： 外文期刊

作者： Feng, Zhike 1 ; Chen, Xiaojiao 1 ; Bao, Yiqun 2 ; Dong, Jiahong 3 ; Zhang, Zhongkai 3 ; Tao, Xiaorong 1 ;

作者机构： 1.Nanjing Agr Univ, Dept Plant Pathol, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

3.Yunnan Acad Agr Sci, Inst Biotechnol & Genet Resources, Yunnan Prov Key Lab Agri Biotechnol, Kunming 650223, Yunnan, Peoples R China

关键词： actin filaments;endoplasmic reticulum;intracellular movement;myosin motor;nucleocapsid;Tomato spotted wilt tospovirus

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

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收录情况： SCI

摘要： A number of viral proteins from plant viruses, other than movement proteins, have been shown to traffic intracellularly along actin filaments and to be involved in viral infection. However, there has been no report that a viral capsid protein may traffic within a cell by utilizing the actin/endoplasmic reticulum (ER) network. We used Tomato spotted wilt tospovirus (TSWV) as a model virus to study the cell biological properties of a nucleocapsid (N) protein. We found that TSWV N protein was capable offorming highly motile cytoplasmic inclusions that moved along the ER and actin network. The disruption of actin filaments by latrunculin B, an actin-depolymerizing agent, almost stopped the intracellular movement of N inclusions, whereas treatment witha microtubule-depolymerizing reagent, oryzalin, did not. The over-expression of a myosin XI-K tail, functioning in a dominant-negative manner, completely halted the movement of N inclusions. Latrunculin B treatment strongly inhibited the formation of TSWV local lesions in Nicotiana tabacum cv Samsun NN and delayed systemic infection in N. benthamiana. Collectively, our findings provide the first evidence that the capsid protein of a plant virus has the novel property of intracellular trafficking. The findings add capsid protein as a new class of viral protein that traffics on the actin/ER system.

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