Comparison of Lisianthus (Eustoma grandiflorum) Cultivars Based on the Selected Regeneration Media Using Anther Culture
文献类型： 外文期刊
作者： Zhou, Xuhong 1 ; Mo, Xijun 2 ; Qu, Suping 2 ; Tian, Min 2 ; Wu, Xuewei 3 ; Wu, Min; Li, Jinze; Luo, Ying 1 ; Wang, Jih 1 ;
作者机构： 1.Kunming Univ Sci & Technol, Fac Environm Sci & Engn, Chenggong 650500, Kunming, Peoples R China
2.Yunnan Acad Agr Sci, Key Lab Flower Breeding Yunnan Prov, Natl Engn Res Ctr Ornamental Hort, Flower Res Inst,Flower Engn Ctr Yunnan Prov, Kunming North Suburb 650205, Yunnan, Peoples R China
3.Yunnan Acad Agr Sci, Key Lab Flower Breeding Yunnan Prov, Natl
关键词： calli;genotype;plant regeneration
期刊名称：HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY （ 影响因子：1.842； 五年影响因子：2.083 ）
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收录情况： SCI
摘要： Double haploids in lisianthus cultivars were produced using anther culture. The anther of 'Art Peach' was cultured on the Murashige and Skoog (MS) medium with 3% sucrose with auxin and cytokinin. The highest frequency of calli developed from anthers (100%) and green plantlets per100 calli (60 regenerants) was observed on the medium containing 8 mgL~(-1)N6 benzyladenine (BA) and 0.1mg-L~(-1) naphthaleneacetic acids (NAA). When anthers were cultured on the medium supplemented with 2 mg L~(-1) BA and 0.5 mg-L~(-1) NAA, 33.3% of calli formed plantlets. Using the anther culture, callus induction and plantlets regeneration in five cultivars were developed differently, suggesting that the genotype might predetermined anther culture response. These resultsindicate that a highly efficient new microprogation technique can be provided by anther culture in vitro for lisianthus. Anther culture in vitro can provide a highly efficient new microprogation technique for lisianthus.
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