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Isolation and Characterization of a Novel Lectin from the Edible Mushroom Stropharia rugosoannulata

文献类型： 外文期刊

作者： Zhang, Weiwei 1 ; Tian, Guoting 3 ; Geng, Xueran 1 ; Zhao, Yongchang 3 ; Tzi Bun Ng 4 ; Zhao, Liyan 5 ; Wang, Hexiang 1 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China

3.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming 650223, Peoples R China

4.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

5.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

关键词： lectin;Stropharia rugosoannulata;hemagglutinating activity;antiproliferative activity

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

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收录情况： SCI

摘要： To date, only a few steroids have been isolated from the mushroom Stropharia rugosoannulata which can be cultivated. In this paper, a novel lectin (SRL) with a molecular weight of 38 kDa, and a unique IKSGVYRIVSWQGALGPEAR N-terminal sequence was isolated from S. rugosoannulata, which represents the first protein isolated from the mushroom. The purification methods included (NH4)(2)SO4 precipitation, ion exchange chromatography on CM-cellulose, Q-Sepharose, and SP-Sepharose, and gel-filtration on Superdex-75. The lectin was adsorbed on all three types of ion exchangers and was purified more than 450-fold. The lectin was stable below 70 degrees C (with half of the activity preserved at 80 degrees C), and in the presence of NaOH and HCl solutions up to a concentration of 12.5 mM and 25 mM, respectively. The hemagglutinating activity of SRL was inhibited by inulin. Cd2+ and Hg2+ ions strongly reduced the hemagglutinating activity at concentrations from 1.25 mM to 10 mM. SRL exhibited anti-proliferative activity toward both hepatoma Hep G2 cells and leukemia L1210 cells, with an IC50 of 7 mu M and 19 mu M, respectively. The activity of HIV-1 reverse transcriptase could also be inhibited by SRL, with an IC50 of 10 mu M.

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[1]Isolation and characterization of a novel thermostable lectin from the wild edible mushroom Agaricus arvensis. Zhao, J. K.,Wang, H. X.,Zhao, J. K.,Wang, H. X.,Zhao, Y. C.,Li, S. H.,Ng, T. B.. 2011

[2]A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica. Zhao, Shuang,Zhao, Yongchang,Li, Shuhong,Ng, Tzi Bun,Zhao, Shuang,Zhao, Jingkun,Zhang, Guoqing,Wang, Hexiang.

[3]Cloning and sequencing of a lectin protein gene from the roots of Sophora flavescens. Ma, ZG,Yan, B,Huang, XQ,Wang, LX,Zeng, ZK. 2001

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