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Use of Digital Gene Expression to Discriminate Gene Expression in Different Developmental Stages of Rosa chinensis 'Pallida'

文献类型： 外文期刊

作者： Yan, H. J. 1 ; Yang, J. K. 2 ; Zhang, H. 1 ; Li, S. B. 1 ; Zhang, T. 1 ; Wang, Q. G. 1 ; Zhou, N. N. 1 ; Chen, M. 1 ; Tang, 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Flower Res Inst, Kunming 650205, Yunnan, Peoples R China

2.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China

关键词： Rosa chinensis 'Pallida';digital gene expression;scent-related gene;qRT-PCR

期刊名称：VI INTERNATIONAL SYMPOSIUM ON ROSE RESEARCH AND CULTIVATION

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收录情况： SCI

摘要： Rosa chinensis 'Pallida' (Rosa L.) is one of the most important ancient rose cultivars originating in China. It contributed the 'tea scent' trait to modern roses. However; very little information available on the gene regulatory networks was involvedin scent biosynthesis and metabolism in Rosa. In this study, digital gene expression (DGE) profiling was used to measure the gene expression in flower bud, bloom and senescent development stages. A total of 16,223 transcripts exhibited significant expression change among the three libraries. KEGG pathways analysis showed that specific enrichment of genes was observed for pathways involved in phenylpropanoid and terpenoid backbone biosynthesis in the three developmental stages. Five transcripts encodingputative proteins related to scent synthesis were identified and their expressions were validated using qRT-PCR. The study provides the foundation for scent-related gene discovery and development of functional molecular markers in roses.

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