Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology
文献类型： 外文期刊
作者： Chu, Bowen 1 ; Shi, Yao 1 ; Li, Zhimin 1 ; Tian, Hao 1 ; Li, Wanyi 1 ; Wang, Yuanzhong 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Peoples R China
2.Yunnan Coll Tradit Chinese Med, Coll Tradit Chinese Med, Kunming 650500, Peoples R China
3.Yunnan Tech Ctr Qual Chinese Mat Med, Kunming 650200, Peoples R China
期刊名称：JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY （ 影响因子：2.193； 五年影响因子：2.4 ）
ISSN：
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收录情况： SCI
摘要： Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33 degrees C, and ratio of solvent to raw material of 10.21:1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values (98.61 +/- 0.61).
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